A new mathematical model for relative quantification in real-time RT-PCR.

By M. W. Pfaffl. Nucleic Acids Research, 2001

33,883 citations recorded by the source corpus.

Subfields: Epidemiology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/dea08357457ed59f599e5bf2ff3e24f5bdd42005

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Epidemiology

In Molecular Biology

Citations in the corpus

Phytochrome-mediated inhibition of shade avoidance involves degradation of growth-promoting bHLH transcription factors. (2007, methodology): “The expression of all samples relative to Col-0 at dawn was determined according to the method described by Pfaffl (2001), using ACTIN2 as a reference gene.”
Copper‐dependent iron transport in coastal and oceanic diatoms (2006, methodology): “Under these conditions, the 22DDCT method provides a reliable calculation of fold change in transcript abundance between two samples (Pfaffl 2001).”
Rapid disuse and denervation atrophy involve transcriptional changes similar to those of muscle wasting during systemic diseases (2007, methodology): “Differences in gene expression were evaluated by a relative quantification method, as described by Pfaffl et al. ( 44 ).”
IL-6 mediates hypoferremia of inflammation by inducing the synthesis of the iron regulatory hormone hepcidin. (2004, neutral): “The relative expression in each sample was calculated by a mathematical method based on the real-time PCR efficiencies (22) using as references mRNA mouse β -actin for murine cells and tissues and human G3PDH for human cells.”
Exon circularization requires canonical splice signals. (2015, positive): “the DDCt-based method (Pfaffl, 2001), and presented as the log2-fold difference in circRNA expression.”
Circulating microRNAs in plasma of patients with gastric cancers (2010, methodology): “Fold change in the gene was calculated by the equation 2-DDCt (Livak and Schmittgen, 2001; Pfaffl, 2001).”
Phosphorylation‐dependent degradation of c‐Myc is mediated by the F‐box protein Fbw7 (2004, methodology): “The relative amounts of each target mRNA were calculated as described (Pfaffl, 2001).”