Circulating microRNAs in plasma of patients with gastric cancers

By M. Tsujiura, D. Ichikawa, S. Komatsu, A. Shiozaki, H. Takeshita, T. Kosuga, H. Konishi, R. Morimura, K. Deguchi, H. Fujiwara and 2 more. British Journal of Cancer, 2010

625 citations recorded by the source corpus.

Background:We examined plasma microRNA (miRNA) concentrations from patients with gastric cancers (GCs) to assess their clinical application for diagnosing and monitoring diseases.Methods:We initially investigated the appropriateness of plasma miRNA assay, and then compared plasma miRNA results with the expressions in cancer tissues from eight GC patients, and also compared plasma miRNAs between pre- and post-operative paired samples from 10 GC patients. Then, plasma miRNAs (miR-17-5p, miR-21, miR-106a, miR-106b and let-7a) were analysed in 69 GC patients and 30 healthy volunteers in total.Results:The initial analysis showed that miRNAs were stable and detectable in all plasma samples, and the plasma miRNA levels reflected the tumour miRNAs in most cases. The levels of these miRNAs were significantly reduced in post-operative samples. In large-scale analysis, the plasma concentrations of miRNAs (miR-17-5p, miR-21, miR-106a, miR-106b) were significantly higher in GC patients than controls (P=0.05, 0.006, 0.008 and <0.001 respectively), whereas let-7a was lower in GC patients (P=0.002). The values of the area under the receiver-operating characteristic curve were 0.721 for the miR-106b assay and 0.879 for the miR-106a/let-7a ratio assay.Conclusion:Detection of circulating miRNAs might provide new complementary tumour markers for GC.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/bb8e3ccbdfeba949c12308ce6f978c69e1cae42a

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In Cancer Research

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1. Andrew F. Hill
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Citations in the corpus

• Blood cell origin of circulating microRNAs: a cautionary note for cancer biomarker studies (2011, background): “A similar concern may be raised for many other studies where circulating miRNAs that are expressed highly in blood cells have been reported as cancer biomarkers (9-11, 15, 16, 20, 23-32).”
• Exosomes provide a protective and enriched source of miRNA for biomarker profiling compared to intracellular and cell-free blood (2014, background): “Expression profiling of miRNA associated with disease has been explored where the majority of researchers have used peripheral blood (5,6) or cell-free serum or plasma (7,8).”
• Influence of storage condition on exosome recovery (2016, positive): “In addition to these pathogenic roles, protein and microRNA profile of exosomes have been regarded as a promising biomarker of diseases [5,6,8,33,35].”
• Circulating MicroRNAs as a Novel Class of Diagnostic Biomarkers in Gastrointestinal Tumors Detection: A Meta-Analysis Based on 42 Articles (2014, background): “Subsequently, miRNAs as molecular markers have attracted much attention in cancer diagnosis [20,21,44,54,67].”