Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

By R. M. Bowers, Nikos Kyrpides, Ramunas Stepanauskas, Miranda Harmon-Smith, Devin F R Doud, T. Reddy, Frederik Schulz, J. Jarett, A. Rivers, Emiley A. Eloe-Fadrosh and 44 more. Nature Biotechnology, 2017

2,110 citations recorded by the source corpus.

We present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a Metagenome-Assembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Gene Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.

Subfields: Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/de6b66013fcebf03d997d28b384497a1d3733670

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Citations in the corpus

CheckM2: a rapid, scalable and accurate tool for assessing microbial genome quality using machine learning (2022, methodology): “…performance of ML models, predictions on simulated genomes were divided into four groups based on MIMAG completeness and contamination standards 12 ( high quality : >90% complete, <5% contaminated; medium quality : 50%-90% complete, <10% contaminated; low quality : <50% complete, <10%…”
VIBRANT: automated recovery, annotation and curation of microbial viruses, and evaluation of viral community function from genomic sequences (2020, positive): “These quality analyses were determined to best reflect established completeness metrics for both bacteria and viruses [72, 73].”
A practical guide to amplicon and metagenomic analysis of microbiome data (2020, background): “…about metagenome sequence (Field et al., 2008; Yilmaz et al., 2011), minimum information about a single ampli ﬁ ed genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea (Bowers et al., 2017), and minimum information about an uncultivated virus genome (Roux et al., 2019).”
The European Nucleotide Archive in 2019 (2019, background): “• MIMAGs, for metagenome-assembled genomes (4); • MISAGs, for environmental single-cell amplified genomes (4); • MIUVIG, for environmental/uncultivated virus genomes (8).”
Genomes OnLine Database (GOLD) v.8: overview and updates (2020, methodology): “GOLD APs follow the standards set by the Genomic Standards Consortium (GSC) including Minimum Information about a Single Amplified Genome (MISAG) and Minimum Information about a Metagenome-Assembled Genome (MIMAG) (25), facilitating their community wide adoption.”