Neural stem cells and neurospheres—re-evaluating the relationship

By B. Reynolds, R. Rietze. Nature Methods, 2005

693 citations recorded by the source corpus.

Subfields: Cellular and Molecular Neuroscience, Developmental Neuroscience, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/dd5462b4891a5a416729ecb1b3867bd179c4d843

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In Cellular and Molecular Neuroscience

In Developmental Neuroscience

In Molecular Biology

Citations in the corpus

The effect of substrate stiffness on adult neural stem cell behavior. (2009, background): “The majority of the observed proliferation can likely be attributed to progenitors, since neurosphere culture in proliferation media has demonstrated that uncommitted neural stem cells make up less than 1% of the total population [54].”
Origin of Oligodendrocytes in the Subventricular Zone of the Adult Brain (2006, background): “…growth factors, which may modify the normal differentiation program of neural progenitors (Doetsch et al., 2002; Gabay et al., 2003; Santa-Olalla et al., 2003; Hack et al., 2004; Reynolds and Rietze, 2005 In contrast, PSA-NCAM ϩ cells present at the surface of the CC did not colabel with Tuj1 (F–”
Eyes Wide Open: A Critical Review of Sphere-Formation as an Assay For Stem Cells (2011, background): “Based on modeling of predicted and actual serial sphereforming capacity, it has been proposed that the neurosphere assay overestimates stem cell frequency by an order of magnitude (Reynolds and Rietze, 2005).”
Origin of new glial cells in intact and injured adult spinal cord. (2010, background): “Restricted progenitor cells have been shown to give rise to primary neurospheres with limited self-renewal and/or differentiation potential (Reynolds and Rietze, 2005; Wu et al., 2005).”
Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens. (2009, background): “Short-lived progenitor cells also proliferate in suspension culture, and true clonal analysis is hampered by sphere aggregation (Suslov et al., 2002; Reynolds and Rietze, 2005; Singec et al., 2006).”
FoxO3 regulates neural stem cell homeostasis (2009, positive): “Although neurospheres can fuse, which can limit the interpretation of these experiments (Reynolds and Rietze, 2005), these results confirm that FoxO3 regulates the NSC pool.”