A PCR primer bank for quantitative gene expression analysis.

By Xiaowei Wang, Brian Seed. Nucleic Acids Research, 2003

906 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/d9db84228f2c145fe4eaba0f2ee47d871f00e9f5

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Citations in the corpus

Differential expression of (dihydro)ceramide synthases in mouse brain: oligodendrocyte-specific expression of CerS2/Lass2 (2008, positive): “Some of the primer sequences were obtained from PrimerBank (Wang and Seed 2003).”
QuantPrime – a flexible tool for reliable high-throughput primer design for quantitative PCR (2008, background): “Another example is the PrimerBank [12], which covers primer pairs for human and mouse transcripts, which could be useful for high-throughput purposes (due to strict design criteria), even though amplicon sizes vary.”
PrimerBank: a PCR primer database for quantitative gene expression analysis, 2012 update (2011, methodology): “This new algorithm is similar to our previous algorithm (11), with some modifications.”
Quantitative real-time RT-PCR--a perspective. (2005, methodology): “A recent report describes a useful algorithm for the identiﬁcation of sequence-speciﬁc primers, which applies the highest ﬁlter stringency to residues at the 3 (cid:1) -end of the primer and to adventitious matches with abundant non-coding RNA (Wang & Seed 2003).”
PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification (2009, methodology): “So, for the PrimerBank primer design, various ﬁlters for cross-reactivity were used to reduce nonspeciﬁc ampliﬁcation (29).”
Cellular stress conditions are reflected in the protein and RNA content of endothelial cell-derived exosomes (2012, methodology): “Primer sequences were taken from the Harvard Primer Databank (31) and synthesized by Sigma-Aldrich.”
Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. (2008, methodology): “Primer sequences for qPCR were taken from PrimerBank (http://pga.mgh.harvard.edu/primerbank/index.html) (Wang and Seed, 2003) and are listed in Table S1.”
A Nurr1/CoREST transrepression pathway attenuates neurotoxic inflammation in activated microglia and astrocytes (2009, neutral): “The sequences of qPCR primers used for mRNA quantification in this study were obtained from PrimerBank (Wang and Seed, 2003).”
Quantification of mRNA using real-time RT-PCR (2006, background): “PrimerBank lists more assays (180,000) but only a small percentage of the assays designed using uPrime”
The real-time polymerase chain reaction. (2006, background): “This problem can be avoided by designing the two PCR primers to hybridize to different exons, hence having at least one intron in between, or designing one of the primers to span across an exon/exon boundary (Wang and Seed, 2003).”