PrimerBank: a PCR primer database for quantitative gene expression analysis, 2012 update
By Xiaowei Wang, Athanasia Spandidos, Huajun Wang, Brian Seed. Nucleic Acids Res., 2011
673 citations recorded by the source corpus.
Optimization of primer sequences for polymerase chain reaction (PCR) and quantitative PCR (qPCR) and reaction conditions remains an experimental challenge. We have developed a resource, PrimerBank, which contains primers that can be used for PCR and qPCR under stringent and allele-invariant amplification conditions. A distinguishing feature of PrimerBank is the experimental validation of primer pairs covering most known mouse genes. Here, we describe a major update of PrimerBank that includes the design of new primers covering 17 076 and 18 086 genes for the human and mouse species, respectively. As a result of this update, PrimerBank contains 497 156 primers (an increase of 62% from the previous version) that cover 36 928 human and mouse genes, corresponding to around 94% of all known protein-coding gene sequences. An updated algorithm based on our previous approach was used to design new primers using current genomic information available from the National Center for Biotechnology Information (NCBI). PrimerBank primers work under uniform PCR conditions, and can be used for high-throughput or genome-wide qPCR. Because of their broader linear dynamic range and greater sensitivity, qPCR approaches are used to reanalyze changes in expression suggested by exploratory technologies such as microarrays and RNA-Seq. The primers and all experimental validation data can be freely accessed from the PrimerBank website, http://pga.mgh.harvard.edu/primerbank/.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/d790d15cb5686bfec43bf2bd444e1177101f9b4b
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
Citations in the corpus
- Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets (2019, positive): “Primers were designed using Primer Bank (Wang et al., 2012).”
- An overview of calf diarrhea - infectious etiology, diagnosis, and intervention (2014, background): “Nevertheless, there is a size limit for the PCR product (usually less than 200 bp) in order to maintain stable sensitivity [141].”