Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence

By E. Semenova, M. Jore, K. Datsenko, Anna Semenova, E. Westra, B. Wanner, J. van der Oost, Stan J. J. Brouns, Konstantin Severinov. 2011

719 citations recorded by the source corpus.

Subfields: Cardiology and Cardiovascular Medicine, Molecular Biology

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Citations in the corpus

Biology and Applications of CRISPR Systems: Harnessing Nature's Toolbox for Genome Engineering. (2016, neutral): “coli Cascade complex, at the 1 to 5 and 7 to 8 position of the spacer, is enough to decrease target DNA binding and subsequent interference (Semenova et al., 2011).”
CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation. (2011, background): “The process is initiated at the seed sequence adjacent to the PAM and then propagated along the protospacer region in a 5′→3′ direction over the complete protospacer region (97, 119).”
Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation (2014, background): “The observed prevalence of PAM mutation as a mechanism of viral escape from CRISPR/Cas9 targeting (43, 44) has presumably spurred the evolution of Cas9 proteins with a”
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity (2013, neutral): “…2 and Supplementary Figures S4-S6) show that when only single-mutant sequences are considered, the six to eight base pairs closest to the PAM are generally highly specified and the non-PAM end is poorly specified under enzyme-limiting conditions, consistent with previous findings 11, 12, 17-19 .”
Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria (2012, background): “Target recognition is dependent on the PAM and governed by the seed crRNA sequence located at the 5′ end of the spacer region (24).”
CRISPR Gene Therapy: Applications, Limitations, and Implications for the Future (2020, background): “Many features in an sgRNA determine specificity including the seed sequence (a 10–12 bp region proximal to PAM on 3 of spacer sequence) (29, 53), GC content (54, 55), and modifications such as 5 truncation of the sgRNA (56).”
CRISPRTarget (2013, background): “38-40 For type I and II systems, protospacer adjacent motifs (PAM) are required for recognition, 23,25,37,39,41,42 and a short seed sequence within the match is required in particular subtypes.”
CRISPR-Cas Systems: RNA-mediated Adaptive Immunity in Bacteria and Archaea (2013, background): “…of the type II tracrRNA-based processing mechanism Delcheva et al. 2011 Defining limits of type I DNA targeting specificity Gudbergsdottir et al. Semenova et al. 2011 Crystal structure of a complex of Cas6 and single stranded pre-crRNA Wang et al. 2011 Reclassification of Cas proteins and…”
Identifying and visualizing functional PAM diversity across CRISPR-Cas systems (2016, background): “…flanking sequence (called the protospaceradjacent motif or PAM) initially drives DNA interrogation by the Cas effector proteins prior to DNA unwinding and base pairing with the loaded guide RNA (Marraffini and Sontheimer, 2010; Semenova et al., 2011; Sternberg et al., 2014;Westra et al., 2012).”
The Biology of CRISPR-Cas: Backward and Forward. (2018, positive): “For both CRISPR classes, escape mutations are typically located in the PAM sequence or the seed region of the protospacer and lead to inefﬁcient cleavage by the Cas protein machinery (Deveau et al., 2008; Semenova et al., 2011).”