Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

By G. Gasiunas, Rodolphe Barrangou, Philippe Horvath, V. Šikšnys. Proceedings of the National Academy of Sciences of the United States of America, 2012

2,600 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/7ef7428662edbec19e2d5c302b48708ff78e0a74

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Citations in the corpus

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA (2014, background): “consistent with previous results showing that Asp10 is critical for the cleavage of the noncomplementary DNA strand, and that Cas9 requires Mg2+ ions for the cleavage activity (Gasiunas et al., 2012; Jinek et al., 2012).”
Crystal structure of Cpf1 in complex with guide RNA and target DNA (2016, background): “Fourth, Cpf1 contains the RuvC domain but lacks a detectable second endonuclease domain (Zetsche et al., 2015), whereas Cas9 uses the HNH and RuvC endonuclease domains to cleave the target and non-target DNA strands, respectively (Jinek et al., 2012; Gasiunas et al., 2012).”
Highly Specific and Efficient CRISPR/Cas9-Catalyzed Homology-Directed Repair in Drosophila (2014, background): “Endogenous prokaryotic type II CRISPR immune systems are composed of a single polypeptide nuclease, Cas9, that is guided to target sites by a complex of two small RNAs (Gasiunas et al. 2012; Jinek et al. 2012).”
Identifying and visualizing functional PAM diversity across CRISPR-Cas systems (2016, background): “…their 20–30 nucleotide spacer region to base pair with complementary protospacer sequences, leading the system’s Cas effector proteins to cleave or degrade these targets (Garneau et al., 2010; Gasiunas et al., 2012; Hale et al., 2009; Jinek et al., 2013; Jore et al., 2011; Westra et al., 2012).”
Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA[OPEN] (2015, background): “…generate site-specific DSB, the system minimally requires Cas9 protein and a duplex of CRISPR RNA (crRNA), a transencoded CRISPR RNA (tracrRNA), and the presence of a protospaceradjacent motif (PAM), which flanks the 39 end of the crRNA-targeted sequence (Gasiunas et al., 2012; Cong et al., 2013).”
The CRISPR/Cas9 system for plant genome editing and beyond. (2015, background): “A prerequisite for cleavage is the presence of a conserved protospacer-adjacent motif (PAM) downstream of the target DNA, which usually has the sequence 5′-NGG-3′ (Gasiunas et al., 2012; Jinek et al., 2012) but less frequently NAG (Hsu et al., 2013).”
Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity (2013, methodology): “Mutations of the catalytic residues (D10A in RuvC and H840A in HNH) convert Cas9 into DNA nickases (Cong et al., 2013; Gasiunas et al., 2012; Jinek et al., 2012).”
CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling (2014, background): “…Research 3Department of Brain and Cognitive Sciences 4Department of Biological Engineering 5David H. Koch Institute for Integrative Cancer Research 6Department of Biology 7Harvard-MIT Division of Health Sciences and Technology 8Institute for Medical Engineering and Science 9Department of Chemical…”
Applications of genome editing technology in the targeted therapy of human diseases: mechanisms, advances and prospects (2020, background): “The sgRNA often contains a unique 20 base-pair (bp) sequence designed to complement the target DNA site in a sequence-specific manner, and this must be followed by a short DNA sequence upstream essential for the compatibility with the Cas9 protein used, which is termed the “protospacer adjacent moti”
Repair of strand breaks by homologous recombination. (2013, background): “Evidence that singlestrand nicks or gaps are likewise repaired by HR in mammalian cells comes not only from studies with drugs like camptothecin but also from the use of site-specific nickases, such as meganucleases (Davis and Maizels 2011), zinc finger nucleases (Kim et al. 2012; Ramirez et al. 201”