Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae

By S. Ficarro, M. McCleland, P. Stukenberg, D. Burke, M. Ross, J. Shabanowitz, D. Hunt, F. White. Nature Biotechnology, 2002

1,576 citations recorded by the source corpus.

Subfields: Cell Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/d69a6f81ddcf331ab57fc8b5d824d7249456ef9f

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Citations in the corpus

FAK phosphorylation sites mapped by mass spectrometry (2005, methodology): “The resulting peptides were enriched for phosphopeptides by immobilized metal affinity chromatography (IMAC) ( Ficarro et al., 2002 ) and then analyzed by high performance liquid chromatography (HPLC) interfaced to electrospray ionization on tandem mass spectrometers (LCQ Deca XP or LTQ-FT, Thermo E”
Mass spectrometry–based proteomics turns quantitative (2005, background): “blocked acidic groups with the same reaction to prevent unspecific binding while purifying phosphopeptides on immobilized metal affinity column”
Understanding storage starch biosynthesis in plants: a means to quality improvement (2006, background): “Recent advances in the use of MS and phosphopeptide derivatization techniques to identify phosphorylated sites raise the possibility of following posttranslational modifications (Ficarro et al. 2002; Qian et al. 2003; Wolschin et al. 2005), which can then be linked to the dynamic assembly of protein”
Intrinsic Disorder Is a Common Feature of Hub Proteins from Four Eukaryotic Interactomes (2006, background): “Interestingly, this prediction coincides with four observed serine/threonine phosphorylation sites [37].”
Proteomics by mass spectrometry: approaches, advances, and applications. (2009, background): “The IMAC selectivity and specificity can be altered with different buffer conditions, such as pH, salt concentration (112), buffer composition, and the presence of detergents (113).”
A proteomics approach to understanding protein ubiquitination (2003, methodology): “To characterize protein phosphorylation several strategies have been developed to capture modified peptides and determine their identity on a large scal”
Quantitative Phosphoproteomics of Early Elicitor Signaling in Arabidopsis*S (2007, background): “Recent advances in selective purification of phosphopeptides have made it possible to overcome these limitations and identify phosphorylation sites in large numbers of proteins (18, 22 , 23).”
From genomics to proteomics (2003, background): “Initial efforts to gauge proteome-wide regulatory events in single experiments have been directed at the yeast phosphoproteom”
Photosynthesis (2016, positive): “Polypeptide patterns obtained after Laemmli (19) are shown in Figs.”