Efficient In Vivo Genome Editing Using RNA-Guided Nucleases

By Woong Y. Hwang, Yanfang Fu, Deepak Reyon, Morgan L. Maeder, S. Tsai, Jeffry D. Sander, Randall T. Peterson, J. Yeh, J. Keith Joung. Nature Biotechnology, 2013

2,554 citations recorded by the source corpus.

In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator–like effector nucleases.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/d3e9f785d3a0ce7e25cc50e8ca105cfca082785b

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In Molecular Biology

Citations in the corpus

Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair (2014, methodology): “sgRNAs and Cas9 mRNA were generated as described previously (Hwang et al. 2013b).”
Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients. (2013, background): “Recently, several groups have demonstrated the use of the CRISPR/Cas9 system for genome engineering in various species (Chang et al., 2013; Cho et al., 2013; Cong et al., 2013; Friedland et al., 2013; Hou et al., 2013; Hwang et al., 2013; Jinek et al., 2013; Li et al., 2013; Mali et al., 2013; Nekra”
Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA (2014, background): “2013), model organisms (Cho et al. 2013b; Dickinson et al. 2013; Friedland et al. 2013; Gratz et al. 2013; Hwang et al. 2013; Li et al. 2013a; Li et al. 2013c; Wang et al. 2013; Yang et al. 2013), plants (Li et al.”
Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans (2014, background): “…in a wide range of organisms (Wang et al. 1999; Chiu et al. 2013; Cho et al. 2013; Dicarlo et al. 2013; Friedland et al. 2013; Gratz et al. 2013; Hwang et al. 2013; Jiang et al. 2013; Katic and Großhans 2013; Li et al. 2013; Lo et al. 2013; Nekrasov et al. 2013; Kim et al. 2014; Zhao et al.…”
Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system (2013, methodology): “The mutagenesis efficiencies in these two studies are ∼24–60% (15) and ∼35% (16), respectively.”
Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease (2013, background): “E-mail: oconnorgiles@wisc.edu Genetics, Vol. 194, 1029–1035 August 2013 1029 eukaryotic genomes, including cultured mammalian cells, human stem cells, and yeast (Cho et al. 2013; Cong et al. 2013; Dicarlo et al. 2013; Ding et al. 2013; Jinek et al. 2013; Mali et al. 2013).”