Circularly permuted green fluorescent proteins engineered to sense Ca2+

By Takeharu Nagai, A. Sawano, E. Park. Proceedings of the National Academy of Sciences of the United States of America, 2001

965 citations recorded by the source corpus.

Subfields: Biophysics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/d33f9ebdfc1507221eb8a790c0bdcd3dbb3aa047

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Citations in the corpus

Genetic dissection of neural circuits. (2008, positive): “GECIs have been used in combination with two-photon microscopy in Drosophila (Wang et al., 2003, 2004) (Figures 6E–6G) and mice (Hasan et al., 2004; Garaschuk et al., 2007).”
Fluorescent proteins and their applications in imaging living cells and tissues. (2010, methodology): “This GES design strategy resulted in a number of calcium sensors (306, 309, 322, 407, 424, 428) and was also applied to create sensors of hydrogen peroxide (39), phosphorylation (205), and membrane potential (119, 217), although the latter two directions have not yet produced effective GES.”
Superoxide Flashes in Single Mitochondria (2008, positive): “As would be expected of a fluorescent protein-based indicator (Belousov et al., 2006; Nagai et al., 2001 ), cpYFP emission is brighter in an alkaline environment, such as that within the mitochondrial matrix (pH � 8.0, Figure S1F).”
NCLX is an essential component of mitochondrial Na+/Ca2+ exchange (2009, methodology): “, Ca-sensitive wavelength (12), presented as F430 (1-F/F0)] and 485 nm [i.”
Calcium at the Crossroads of Signaling Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.002899. (2002, background): “For example, insertions of calmodulin into green fluorescent protein derivatives, such as the newly developed “pericam” proteins, can allow fast line scan imaging of rapid, highly loclalised Ca2 elevations (Nagai et al., 2001).”
Imaging interorganelle contacts and local calcium dynamics at the ER-mitochondrial interface (2010, positive): “To make this measurement feasible, a Ca-sensitive fluorescent protein, ratiometric pericam (pcm) (Nagai et al., 2001), was placed in the OMM-targeted FKBP12 instead of mRFP (Figure 5A), and mRFP was used instead of CFP in the ER-targeted FRB domain to avoid spectral overlap (Figure 5A).”