Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics

By Hari Shroff, C. Galbraith, J. Galbraith, Eric Betzig. Nature Methods, 2008

866 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Biophysics, Structural Biology

View Paper: https://www.semanticscholar.org/paper/d07995012cda2a8709b256ace45f9792c0ecb0c1

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Citations in the corpus

Quantum metrology and its application in biology (2014, methodology): “A number of particularly powerful techniques were developed for photoswitchable fluorescent probes, including stimulated emission depletion (STED) microscopy [92, 93], photoactivated localization microscopy (PALM) [94], and stochastic optical reconstruction microscopy (STORM) [95].”
Fluorophore localization algorithms for super-resolution microscopy (2014, background): “…fluorophore positions; the resolution of the reconstruction, and its fidelity to the underlying specimen, depends on the density of the labels (the Nyquist criterion 11 ) and the process of reconstructing the final image 12 as well as the precision with which the fluorophores are localized 12–14 .”
Fast three-dimensional super-resolution imaging of live cells (2011, background): “This requirement is best characterized by the Nyquist criterion which equates the image resolution to 2/(localization density) 1/D , where D = 1, 2, or 3 for one-dimensional (1D), 2D or 3D imaging, respectivel”
FtsZ in Bacterial Cytokinesis: Cytoskeleton and Force Generator All in One (2010, background): “There are many technical hurdles, but the study of the structure of the Z ring would seem to be one of the most attractive applications for PALM.”
Microscopy and its focal switch (2008, methodology): “However, the registration of the data and the correct image assembly require more cautio”
TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation (2009, methodology): “We achieved this with a combination of three techniques: a modified high-speed version of photoactivated localization microscopy (hsPALM”
Fluorescent probes for super-resolution imaging in living cells (2008, background): “Recently, PALM and FPALM have been extended to live-cell imagin”