TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation
By Björn F. Lillemeier, Manuel A. Mörtelmaier, M. Forstner, J. Huppa, J. Groves, Mark M. Davis. Nature Immunology, 2009
643 citations recorded by the source corpus.
The organization and dynamics of receptors and other molecules in the plasma membrane are not well understood. Here we analyzed the spatio-temporal dynamics of T cell antigen receptor (TCR) complexes and linker for activation of T cells (Lat), a key adaptor molecule in the TCR signaling pathway, in T cell membranes using high-speed photoactivated localization microscopy, dual-color fluorescence cross-correlation spectroscopy and transmission electron microscopy. In quiescent T cells, both molecules existed in separate membrane domains (protein islands), and these domains concatenated after T cell activation. These concatemers were identical to signaling microclusters, a prominent hallmark of T cell activation. This separation versus physical juxtapositioning of receptor domains and domains containing downstream signaling molecules in quiescent versus activated T cells may be a general feature of plasma membrane–associated signal transduction.
Subfields: Immunology
View Paper: https://www.semanticscholar.org/paper/ac672be297c55dba90585070e6b569fb688f6eec
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In Immunology
Citations in the corpus
- The Immunological Synapse (2014, background): “It has been argued that TCRs are preclustered on T cells, but the ligand-induced clusters have a number of distinct characteristics (79, 114).”
- Probing protein heterogeneity in the plasma membrane using PALM and pair correlation analysis (2011, methodology): “Consequently, PALM has been used to characterize various plasma-membrane proteins, including T-cell receptor and Lat distribution on plasma-membrane sheets from T cell”
- Revitalizing membrane rafts: new tools and insights (2010, methodology): “but high-resolution microscopy methods show complexes with up to 7–20 TCRs, which are manifested as protein islands that are 70–140 nm in diamete”