An economic method for the fluorescent labeling of PCR fragments

By M. Schuelke. Nature Biotechnology, 2000

3,688 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/cf467009bc2b93b76811d8450713328ab757d404

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In Molecular Biology

Citations in the corpus

The evaluation of FHB resistance QTLs introgressed into elite Canadian spring wheat germplasm (2007, methodology): “AAAACGAC) (Schuelke 2000).”
Microsatellite tagging of the leaf rust resistance gene Lr16 on wheat chromosome 2BSc (2005, methodology): “PCR reactions were performed in 96-well PCR plates with 10 ll volumes and included 25 ng of template DNA, 0.5 U of Taq DNA polymerase (Gibco/BRL, Mississauga, Ontario), 1 · PCR buffer (Applied Biosystems, Foster City, California), 1.5 mM MgCl2, 200 lM each dNTP, 20 lM forward primer, 180 lM 6-FAM/HE”
A candidate gene for fire blight resistance in Malus ×robusta 5 is coding for a CC–NBS–LRR (2013, methodology): “The amplification of SSRs was either performed using fluorescently labeled primers in conventional PCR or by using a method described by Schuelke (2000).”
Microsatellite Markers in Plants and Insects. Part I: Applications of Biotechnology (2009, background): “They are PCR-driven, codominant, abundant (representing ~3% of the human genome DNA), relatively evenly distributed throughout the euchromatic part of genomes (Ellegren 2004; Schlotterer 2004), economic, robust, and reproducible (Weber and May 1989; Schuelke 2000).”
Community Resources and Strategies for Association Mapping in Sorghum (2008, positive): “Primer concentrations and cycling conditions were as previously described (Schuelke, 2000) and fl uorescent SSR detection and allele scoring was performed according to Casa et al.”
Construction of an intra-specific sweet cherry (Prunus avium L.) genetic linkage map and synteny analysis with the Prunus reference map (2008, methodology): “These fluorescent fragments were generated using either the IRDye800 or IRDye700 universally labeled M13 (−29) primer in the PCR reaction following the M13-tailed PCR protocol (Schuelke 2000).”
A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.) (2004, methodology): “M13 tailing required adding the M13 sequence (CACGACGTTGTAAAACGAC) to the 5′ end of the forward primer during primer synthesis (Schuelke 2000).”
Association Mapping of Kernel Size and Milling Quality in Wheat (Triticum aestivum L.) Cultivars (2006, methodology): “The 18 markers used for sample selection were analyzed using a three-primer system (Schuelke 2000), including a universal M13 oligonucleotide (TGTAAAACGACGGCCAGT) labeled with one of the fluorescent dyes 6-FAM, VIC, NED, and PET, a special forward primer composed by the concatenation of the M13…”
Advances in molecular marker techniques and their applications in plant sciences (2008, methodology): “Schuelke (2000) introduced a novel procedure in which three primers are used for the amplification of a defined microsatellite locus: a sequence-specific forward primer with M13(–21) tail at its 5 0 end, a sequence-specific reverse primer and the universal fluorescent-labelled M13(–21) primer which…”
Current trends in microsatellite genotyping (2011, methodology): “In particular, M13-tails appear to decrease PCR efficiency, resulting in a need for additional PCR amplification cycles (de Arruda et al. 2010).”
Experimental harvesting of fish populations drives genetically based shifts in body size and maturation (2013, methodology): “Forward primers were extended with an 8-bp tail, complementary to a FAM/NED/VIC/PET fluorescent dye-labeled universal primer following Schuelke (2000) and amplified using a QIAGEN Multiplex PCR kit.”
EST-derived SSR markers in Jatropha curcas L.: development, characterization, polymorphism, and transferability across the species/genera (2011, methodology): “The primers were synthesized (MWG-Biotech AG, Bangalore, India) with an additional 18 base (5′-TGTAAAACGACGGCCAGT-3′) tag at 5′ end to all forward primers as M13 tail (Schuelke 2000).”
Evaluation of Genetic Diversity and Genome‐wide Linkage Disequilibrium among U.S. Wheat (Triticum aestivum L.) Germplasm Representing Different Market Classes (2007, methodology): “Polymerase chain reaction amplifi cations followed the M13-tailed primer PCR method (Schuelke, 2000) with the M13 oligonucleotide labeled with one of the four fl uorescent dyes, 6-FAM, VIC, NED, and PET, added in the reaction mix.”