Discovery of an RNA virus 3'->5' exoribonuclease that is critically involved in coronavirus RNA synthesis.

By Ekaterina Minskaia, Tobias Hertzig, Alexander E. Gorbalenya, V. Campanacci, Christian Cambillau, Bruno Canard, J. Ziebuhr. Proceedings of the National Academy of Sciences of the United States of America, 2006

614 citations recorded by the source corpus.

Subfields: Animal Science and Zoology, Infectious Diseases

View Paper: https://www.semanticscholar.org/paper/cebcd7c50540faab27b4d958179ad3233b8f476a

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Citations in the corpus

Nidovirales: Evolving the largest RNA virus genome (2006, background): “Unlike its arterivirus counterpart (Molenkamp et al., 2000), the N protein of coronaviruses was reported to be essential for efficient sg RNA synthesis and genome replication (Almazan et al., 2004; Schelle et al., 2005).”
Recurrent deletions in the SARS-CoV-2 spike glycoprotein drive antibody escape (2021, background): “Coronaviruses like SARS-CoV-2 slowly acquire substitutions 41 due to a proofreading RNA dependent RNA polymerase (RdRp) (10, 11).”
The Evolution and Emergence of RNA Viruses (2009, background): “Remarkably, the ExoN domain exhibits distant similarities to host cellular proteins of the DEDD superfamily of exonucleases (Snijder et al. 2003; Minskaia et al. 2006) which, among other things, are involved in proofreading and repair.”
Severe Acute Respiratory Syndrome Coronavirus as an Agent of Emerging and Reemerging Infection (2007, neutral): “Orf1a/b nsp1 (180) Expression promoted degradation of host endogenous mRNAs, which may inhibit host protein synthesis and prevented endogenous IFN- mRNA accumulation (167) Induce CCL5, CXCL10 (IP10), and CCL3 expression in human lung epithelial cells via activation of NFB; increases cellular RNA deg”
Emergence of genomic diversity and recurrent mutations in SARS-CoV-2 (2020, background): “…despite Coronaviridae having the unusual capacity amongst viruses of proofreading during nucleotide replication, thanks to the non-structural protein nsp14 exonuclease, which excises erroneous nucleotides inserted by their main RNA polymerase nsp12 (Snijder et al., 2003; Minskaia et al., 2006).”
Viral Mutation Rates (2010, background): “The possibility that the largest RNA viruses, namely, coronaviruses, show low mutation rates is supported by evidence of 3 exonuclease proofreading activity in their replicases (65).”
Coronavirus Pathogenesis (2011, background): “The predicted 30 ! 50 exoribonuclease activity of nsp14 has been characterized biochemically, including identification of residues required for enzyme activity (Chen et al., 2007; Minskaia et al., 2006).”
Hosts and Sources of Endemic Human Coronaviruses (2018, background): “The proofreading and repair mechanisms typical in CoVs may aid the survival and selection of recombinant viruses in populations (Eckerle et al., 2010; Hanada et al., 2004; Minskaia et al., 2006).”