Genomic analysis identifies association of Fusobacterium with colorectal carcinoma.

By A. Kostic, Dirk Gevers, C. Pedamallu, M. Michaud, F. Duke, A. Earl, A. Ojesina, Joonil Jung, A. Bass, J. Tabernero and 8 more. Genome Research, 2012

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The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation.

Subfields: Molecular Biology, Oncology, Surgery

View Paper: https://www.semanticscholar.org/paper/99d3dabe7e0d200c68e3ca5338add65c2245d91b

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Citations in the corpus

Fusobacterium nucleatum Promotes Chemoresistance to Colorectal Cancer by Modulating Autophagy (2017, background): “In agreement with our data in Cohort 1 and previous reports (Castellarin et al., 2012; Kostic et al., 2012), the amount of F.”
Binding of the Fap2 Protein of Fusobacterium nucleatum to Human Inhibitory Receptor TIGIT Protects Tumors from Immune Cell Attack (2015, background): “F. nucleatum Adheres to Various Tumor Cells and Inhibits NK Cell Cytotoxicity F. nucleatum is found in human tumors, particularly colon adenocarcinoma tumors (Castellarin et al., 2012; Kostic et al., 2012).”
Fap2 Mediates Fusobacterium nucleatum Colorectal Adenocarcinoma Enrichment by Binding to Tumor-Expressed Gal-GalNAc (2016, result): “We detected F. nucleatum in human CRC metastases by qPCR (Figure 6A; 10 of 12 tested metastases), consistent with prior preliminary observations (Kostic et al., 2012), but we do not detect fusobacteria in 6 of 7 samples taken from tumor-free liver biopsies (Figure 6A).”
Human Intestinal Lumen and Mucosa-Associated Microbiota in Patients with Colorectal Cancer (2012, result): “Two recently published reports confirmed thses results [46,47].”
The Impact of the Gut Microbiota on Human Health: An Integrative View (2012, neutral): “, 2011), autoimmune demyelination (Kostic et al., 2011), and even behavior (Heijtz et al.”
What is the Healthy Gut Microbiota Composition? A Changing Ecosystem across Age, Environment, Diet, and Diseases (2019, background): “with colorectal cancer [80]; the microbiota of CRC patients were enriched in Fusobacterium spp.”
Role of the Microbiota in Immunity and inflammation (2014, background): “Bacteria of the phylum Fusobacteria are also found at a high frequency residing within the tumor tissue and are important in inducing the traffic of tumor-promoting myeloid cells (Kostic et al., 2012, 2013; Rubinstein et al., 2013).”