Interaction between reactive oxygen species and nitric oxide in drought-induced abscisic acid synthesis in root tips of wheat seedlings

By Zhiguang Zhao, Guo-cang Chen, Cheng-lie Zhang. 2001

165 citations recorded by the source corpus.

Abscisic acid accumulation and oxidative stress are two common responses of plants to environmental stresses. However, little is known about their relationships. The purpose of this article is to investigate the effects of reactive oxygen species and nitric oxide on the plant hormone abscisic acid synthesis in root tips of wheat (Triticum aestivum L.) seedlings under drought stress. Detached root tips were subjected to drought stress by naturally evaporating until 20% of their fresh weights were lost. The activities of superoxide synthases and nitric oxide synthase (EC 1.14.13.39) increased after 20 min of treatment and abscisic acid began to accumulate 60 min later. The induction of abscisic acid by drought was strongly blocked by pretreating the root tips with reactive oxygen species eliminators tiron or ascorbate acid, and with nitric oxide synthase inhibitor N ω -nitro-L-arginine or nitric oxide eliminator 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl 3-oxide. Consistent with these results, reactive oxygen species generators diethyldithiocarbamic acid, xanthine-xanthine oxidase and triazole or nitric oxide donor sodium nitroprusside can also induce abscisic acid accumulation in root tips of wheat seedlings. While potentiated by reactive oxygen species, the effect of sodium nitroprusside on abscisic acid accumulation was blocked by 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl 3-oxide. Based on these results, we suggest that reactive oxygen species and nitric oxide play important roles in drought-induced abscisic acid synthesis in plant, they may be the signals through which the plant can 'sense' the drought condition.

Subfields: Physiology, Plant Science

View Paper: https://www.semanticscholar.org/paper/ccfab88e76a3f2ff48f75de8dd79bc4758dd7203

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Citations in the corpus

Hydrogen peroxide pretreatment induces osmotic stress tolerance by influencing osmolyte and abscisic acid levels in maize leaves (2014, background): “On the other hand, ROSinduced ABA accumulation in root tips of wheat seedlings was recorded by Zhao et al. (2001).”
Nitric oxide as a bioactive signalling molecule in plant stress responses (2007, background): “Moreover, ABA synthesis in wheat roots in response to water deficit was much higher in the presence of NO donors and ROS, which suggests synergistic action of ROS and NO [101].”
Abscisic Acid and Abiotic Stress Tolerance in Crop Plants (2016, background): “Reactive oxygen species could enhance ABA biosynthesis (Zhao et al., 2001).”
Reactive Oxygen Species (ROS) Generation and Detoxifying in Plants (2014, background): “…of abscisic acid (ABA) in mediating Catalase1 gene (CAT1) expression (Guan et al., 2000), thermotolerance (Gong et al., 1998), activation of Ca2+ channels in guard cells (Pei et al., 2000), stomatal closure (Pei et al., 2000; Zhang et al., 2000), and even ABA biosynthesis (Zhao et al., 2001).”
Evidence for the involvement of nitric oxide and reactive oxygen species in osmotic stress tolerance of wheat seedlings: Inverse correlation between leaf abscisic acid accumulation and leaf water loss (2004, background): “Taking into account that ROS can serve as both downstream and upstream signals of ABA (Guan et al. 2000; Pei et al. 2000; Zhang et al 2001; Zhao et al. 2001), the integrative responses of plants to ROS in this context might not be simple.”
Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions (2012, unknown): “Zhao and coworkers [87] reported that ROS play important roles in drought-induced abscisic acid synthesis in plant and suggested that they may be the signals through”
Abscisic Acid and Antioxidant Defense in Plant Cells (2004, unknown): “…showed that in rapid dehydrated root tissues of wheat seedlings, the induction of ABA by dehydration was strongly blocked by ROS scavengers such as Tiron or ASC, and ROS generators diethyldithiocarbamic acid, xanthinexanthine oxidase and triazole also induced ABA accumulation (Zhao et al., 2001).”
Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves. (2002, background): “…rapid dehydrated root tissues of wheat seedlings, the induction of ABA by dehydration was strongly blocked by ROS scavengers such as Tiron or ascorbic acid, and the ROS generators diethyldithiocarbamic acid, xanthine-xanthine oxidase and triazole also induced ABA accumulation (Zhao et al., 2001).”
Exogenously applied nitric oxide enhances the drought tolerance in fine grain aromatic rice (Oryza sativa L.). (2009, background): “Moreover, ABA synthesis in wheat roots in response to water deficit was much higher in the presence of NO donors and ROS, which suggested synergistic action of ROS and NO (Zhao et al. 2001).”
Nitric oxide signalling in plants. (2003, background): “In response to drought stress, an increase in NOS-like activity was observed in wheat seedlings, and ABA accumulation was inhibited by NOS inhibitors (Zhao et al., 2001).”
Cell Signaling during Cold, Drought, and Salt Stress Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.000596. (2002, background): “The observation that ROS may mediate both ABA signaling (see above) and ABA biosynthesis (Zhao et al., 2001) suggests that the feedback regulation of ABA biosynthetic genes by ABA may be mediated in part by ROS through a protein phosphorylation cascade (Figure 5).”