Evidence for the involvement of nitric oxide and reactive oxygen species in osmotic stress tolerance of wheat seedlings: Inverse correlation between leaf abscisic acid accumulation and leaf water loss

By H. Xing, Lingling Tan, L. An, Zhiguang Zhao, Suo-min Wang, Cheng-lie Zhang. Plant growth regulation (Print), 2004

104 citations recorded by the source corpus.

Nitric oxide (NO) and reactive oxygen species (ROS) play important roles in both abscisic acid (ABA) signaling and stress-induced ABA accumulation. However, little is known about their physiological roles in the whole plant. In this study, the effects of NO and ROS on leaf water control and the roles of ABA were determined using wheat (Triticum aestivum L.) seedlings. As compared with the control, osmotic stress reduced leaf water loss (LWL) while it increased leaf ABA content. The effects of osmotic stress on LWL and ABA contents were partially reversed by NO scavengers or NO synthase (NOS) inhibitors. Furthermore, sodium nitroprusside (SNP) at concentrations between 0.01 and 10 mM all reduced LWL efficiently and induced ABA accumulation in a dose-dependent manner. When ABA synthesis was inhibited by fluridone or actidione, the effects of SNP on LWL were partially reversed. These results suggest that NO is involved in leaf water maintenance of wheat seedlings under osmotic stress, and one of the possible mechanisms is by stimulating ABA synthesis. The ROS scavengers used in our experiments had no effects on either LWL or ABA accumulation induced by osmotic stress. However, all ROS induced LWL reduction and ABA accumulation significantly. Hydrogen peroxide had the same effects as SNP on LWL and induced ABA accumulation in a dose-dependent manner but had a maximal effect at 1 mM. Fluridone reversed the effects of H2O2 on both LWL reduction and ABA accumulation, while actidione had no effect. These results suggest that ROS are also involved in leaf water maintenance of wheat seedlings by stimulating ABA biosynthesis, but with a different mechanism to that of NO. The ABA-independent mechanism in NO/ROS regulation of leaf water balance is discussed, in relation to our results.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/b8a745401f8c60c9e4663073f9cff6075b4854e1

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Citations in the corpus

Nitric oxide mediates abscisic acid induced thermotolerance in the calluses from two ecotypes of reed under heat stress (2008, background): “In contrast, there is some evidence that NO serve as upstream signals of stressinduced ABA accumulation under osmotic stress [35] and salt stress [36].”
Effect of osmotic stress on compatible solutes content, membrane stability and water relationsin two maize cultivars (2018, methodology): “Leaf water loss was measured according to the method of Xing et al. (2004).”
Effect of plant growth promoting Pseudomonas spp. on compatible solutes, antioxidant status and plant growth of maize under drought stress (2010, methodology): “Leaf water retention was determined in freshly excised leaves by using bomb meter and leaf water loss was measured according to the method of Xing et al. (2004).”