Homologous recombination in human embryonic stem cells

By T. Zwaka, James A. Thomson. Nature Biotechnology, 2003

693 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/cc89074c6b6732de358034ed136335dc4093a37e

How researchers see this paper

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In Molecular Biology

1. G. Keller
2. Kenneth R. Boheler
3. A. Wobus
4. Kenneth A Kim
5. M. Holmes
6. L. Naldini
7. F. Urnov
8. James A. Thomson

Citations in the corpus

• Immunogenicity of induced pluripotent stem cells (2011, neutral): “…have used standard plasmid vectors to target endogenous genes through homologous recombination in hESCs (Urbach et al., 2004; Costa et al., 2007; Davis et al., 2008; Xue et al., 2009; Irion et al., 2007; Ruby and Zheng, 2009; Zwaka and Thomson, 2003), the targeting efficiency is usually low.”
• Riding the DUBway: regulation of protein trafficking by deubiquitylating enzymes (2006, neutral): “Common oxidant”
• Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery (2007, background): “After delivery of DNA molecules that are largely identical in sequence to the target locus but contain mismatches or intervening sequences, homologous recombination mediates incorporation of the novel sequence into a small fraction of the treated cell”
• Embryonic stem cell differentiation: emergence of a new era in biology and medicine. (2005, background): “With respect to loss-offunction approaches, there is only one report to date of targeting through homologous recombination (Zwaka and Thomson 2003).”
• Embryonic stem cells: prospects for developmental biology and cell therapy. (2005, background): “The first report of successful homologous recombination in hES cells is an important step towards the generation of genetically modified ES cells for transplantations (419).”
• High purity human-induced pluripotent stem cell-derived cardiomyocytes: electrophysiological properties of action potentials and ionic currents. (2011, methodology): “This line was used to generate hiPSC clones that were engineered to exhibit blasticidin resistance in cardiomyocytes by inserting the coding region of the BSD gene encoding blasticidin S deaminase from Aspergillus terreus in-frame downstream of the last exon of the native MYH6 gene coding region thr”
• Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis (2013, background): “Historically, gene targeting in hPSCs has been extremely difficult (2).”
• Somatic cell reprogramming and transitions between pluripotent states: facts, hypotheses, unresolved issues (2010, neutral): “However, the targeting efficiency at this particular locus was 2-fold lower than that reported previously for conventional human ESCs (Zwaka and Thomson, 2003).”