Combined application of antagonist Bacillus amyloliquefaciens and essential oils for the control of peach postharvest diseases

By E. Arrebola, D. Sivakumar, Romina Bacigalupo, L. Korsten. 2010

162 citations recorded by the source corpus.

Bacillus amyloliquefaciens PPCB004 was selected as a potential antagonist to control Botrytis cinerea, Penicillium expansum and Rhizopus stolonifer on peach fruit. The HPLC data of PPCB004 indicated the lipopeptides iturin A, fengycin and surfactin as secondary metabolites. The GC/MS analysis of PPCB004 showed 3-hydroxy-2-butanone as the dominant compound (97.52% of relative peak area). Thyme (TO) and lemongrass (LO) oils showed over 50% and 25% inhibition of radial mycelial growth respectively with 8 ml oil per plate for all pathogens. Combination treatment with both oils failed to increase the percentage inhibition of radial mycelial growth of the pathogens. Combined application of PPCB004 with TO or LO was tested to assess the effectiveness in the control of these pathogens during postharvest storage. The biofilm formation of PPCB004 was significantly higher in LO than TO. LO (6 ml plate �1 ) and PPCB004 completely inhibited the mycelial growth of the pathogens. Fruit inoculation trials with PPCB004 þ LO in NatureFlex modified atmosphere packaging (MAP), showed lower disease incidence and severity at 25 � C for 5 d than treatments with PPCB004 þ MAP, PPCB004 þ TO þ MAP, LO þ MAP, TO þ MAP or stand-alone MAP. On naturally infected fruit, PPCB004 þ LO þ MAP and LO þ MAP treatments retained the total soluble solids/titratable acidity ratio and flesh firmness but failed to stimulate the levels of total phenolic content, phenylalanine ammonia-lyase, b-1,3-glucanase and chitinase activities. Combination of PPCB004 (spray treatment) þ LO (in pad delivery system) in NatureFlex MAP showed absence of disease and off-flavour development, retained the overall appearance and increased the overall acceptance at market shelf conditions (20

Subfields: Cell Biology, Food Science, Plant Science

View Paper: https://www.semanticscholar.org/paper/c63f396b9953944ee54733864d07a801f032f786

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In Cell Biology

1. E. Sadiku
2. P. S. Sellamuthu
3. Reshma B. Nambiar
4. A. Perumal

In Food Science

1. E. Sadiku
2. P. S. Sellamuthu
3. Reshma B. Nambiar
4. A. Perumal

In Plant Science

1. E. Sadiku
2. P. S. Sellamuthu
3. Reshma B. Nambiar
4. A. Perumal

Citations in the corpus

• Use of Essential Oils in Bioactive Edible Coatings: A Review (2011, unknown): “[4 7 ]”
• The efficacy of combined application of edible coatings and thyme oil in inducing resistance components in avocado (Persea americana Mill.) against anthracnose during post-harvest storage (2014, unknown): “Thyme oil directly inhibits pathogen growth and spore germination by affecting the active sites of enzymes and cellular metabolism (Arrebola et al., 2010).”
• Effects of Essential Oil Vapour Treatment on the Postharvest Disease Control and Different Defence Responses in Two Mango (Mangifera indica L.) Cultivars (2017, methodology): “Different volume concentrations of oils were added onto this filter paper disc (1 to 8 μL), and plates with disc containing sterile distilled water served as control (Arrebola et al. 2010).”
• A review on the use of essential oils for postharvest decay control and maintenance of fruit quality during storage (2014, unknown): “Botrytis cinerea Cymbopogon citratus (Lemongrass oil) Arrebola et al. (2010)”