An overview of real-time quantitative PCR: applications to quantify cytokine gene expression.

By A. Giulietti, L. Overbergh, D. Valckx, B. Decallonne, R. Bouillon, Chantal Mathieu. Methods, 2001

1,487 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/c457efd2edadd2b9a1ce4de167b41433925d8ec6

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In Molecular Biology

Citations in the corpus

Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. (2006, methodology): “Real-time PCR analysis has become the most common method for validating the whole-genome microarray data or a smaller set of genes, and molecular diagnostics [3–8].”
IL-17 mRNA in sputum of asthmatic patients: linking T cell driven inflammation and granulocytic influx? (2006, methodology): “Realtime quantitative PCR was performed for interleukin (IL)8, IL-17A, IL-5, CD3γ and β-actin in the ABI prism 7700 Sequence Detector System (Applied Biosystems, Foster City, CA) as described [32].”
Validating internal controls for quantitative plant gene expression studies (2004, methodology): “Real-time PCR technology has removed many of the difficulties associated with quantitative gene expression studies [1], and realtime quantitative RT-PCR (qRT-PCR) is rapidly being adopted as a standard method for in-depth expression studies, including studies of alternative splicing, verification of”
Genome-based PCR Primers for Specific and Sensitive Detection and Quantification of Xylella fastidiosa (2006, neutral): “However, SYBR Green is less specific and reliable than the TaqMan system because SYBR Green binds to any dsDNA, including the specific target DNA, nonspecific PCR products and primer dimers (Giulietti et al., 2001).”
Comparison of reference genes for quantitative real-time polymerase chain reaction analysis of gene expression in sugarcane (2007, background): “GAPDH is an abundant glycolytic enzyme present in most cell types (Giulietti et al., 2001) and has been widely used as a reference gene in RT-PCR experiments in animal and human samples.”
A cryptic promoter in potato virus X vector interrupted plasmid construction (2007, positive): “A relative quantity of Real-time RT-PCR results were calculated using the comparative threshold cycle (CT) method[24].”