Genome-based PCR Primers for Specific and Sensitive Detection and Quantification of Xylella fastidiosa

By M. Francis, Hong Lin, Juan C. Cabrera-La Rosa, H. Doddapaneni, E. Civerolo. European journal of plant pathology, 2006

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DETECTION OF XYLELLA FASTIDIOSA IN OLIVE TREES BY MOLECULAR AND SEROLOGICAL METHODS (2014, methodology): “, 2001), as well real-time and LAMP PCR (Oliveira et al., 2002; Schaad et al., 2002; Francis et al., 2006; Blexine and Child, 2007; Harper et al., 2010; Li et al., 2013; Guan et al., 2013), have been used to detect the bacterium in grapevine, citrus, almond and other hosts.”
Transmission of Xylella fastidiosa by naturally infected Philaenus spumarius (Hemiptera, Aphrophoridae) to different host plants (2017, methodology): “…using a CTAB-based protocol (Loconsole et al. 2014) and SYBR-Greenbased qPCR assays performed using the primers targeting the conserved hypothetical HL protein (Francis et al. 2006); post-amplification melting curve analysis was carried out to assess the specificity of the amplification products.”
IDENTIFICATION OF DNA SEQUENCES RELATED TO XYLELLA FASTIDIOSA IN OLEANDER, ALMOND AND OLIVE TREES EXHIBITING LEAF SCORCH SYMPTOMS IN APULIA (SOUTHERN ITALY) (2013, methodology): “In particular, PCR amplicons were generated by primers targeting the conserved hypothetical HL protein (Francis et al., 2006), the RNA polymerase sigma-70 factor, and the 16S rDNA genes (Rodrigues et al., 2003).”