Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR.

By Mukesh Jain, Aashima Nijhawan, Akhilesh K. Tyagi, J. Khurana. Biochemical and Biophysical Research Communications - BBRC, 2006

1,185 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/1915d67fee2ebb9cda08ee4a4dadb09922097fab

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Citations in the corpus

Validation of Candidate Reference Genes for the Accurate Normalization of Real-Time Quantitative RT-PCR Data in Rice During Seed Development (2010, methodology): “In previous studies, housekeeping gene expression analysis was only carried out during the seed sprouting periods or with different treatments (Kim et al. 2003; Jain et al. 2006).”
An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development (2006, neutral): “Examples such as GAPDH, 18S rRNA and EF1-α have been widely used in RNA blot analyses and are commonly used for real-time RT-PCR in various plant species [2,3,6,7,10,11].”
Genome-wide identification and characterization of the aquaporin gene family in Sorghum bicolor (L.) (2015, background): “These three reference genes are widely used as reference genes in different plant species (Basa et al., 2009; Chandna et al., 2012; Gimeno et al., 2014; Jain et al., 2006; Pinheiro et al., 2011; P.S. Reddy et al. unpublished data).”
Functional analysis of a cold-responsive rice WRKY gene, OsWRKY71 (2016, methodology): “The synthesized first-strand cDNA was used in subsequent PCR reactions as described above with gene-specific primers and control primers for OsUBQ5 (Jain et al. 2006; Supplementary Table S1).”
Overexpression of a cytosolic glyceraldehyde-3-phosphate dehydrogenase gene OsGAPC3 confers salt tolerance in rice (2011, background): “However, a recent study revealed that GAPDH displayed unacceptably high expression variability limiting their use as internal control (Jain et al. 2006).”
Identification of Four Soybean Reference Genes for Gene Expression Normalization (2008, background): “…L.; Nicot et al., 2005), GAPDH, actin and EF1-α in grape (Vitis spp.; Reid et al., 2006), ubiquitin in poplar (Populus spp.; Brunner et al., 2004), and ubiquitin, EF-1α, 18S and 25S rRNA in rice (Oryza sativa L.; Kim et al., 2003; Jain et al., 2006) are considered as useful reference genes.”
Functional complementation of rice blast resistance gene Pi-kh(Pi54) conferring resistance to diverse strains of Magnaporthe oryzae (2011, methodology): “This gene has been used earlier while assessing the expression of genes involved in biotic and abiotic stresses in plants (Jain et al. 2006).”
Genomic Survey and Gene Expression Analysis of the Basic Leucine Zipper Transcription Factor Family in Rice1[W][OA] (2007, methodology): “Isolation of total RNA and quality controls was done as described previously (Jain et al., 2006).”
Identification of suitable reference genes for studying gene expression in cucumber plants subjected to abiotic stress and growth regulators (2011, background): “suitable internal controls for gene expression studies have been identified for rice (Ding et al. 2004; Jain et al. 2006; Kim et al. 2003), Arabidopsis thaliana 344 Mol Breeding (2011) 28:343–357”
Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, positive): “AK101547) was used as an internal control (Jain et al., 2006).”