IL-17 mRNA in sputum of asthmatic patients: linking T cell driven inflammation and granulocytic influx?
By D. Bullens, Els Truyen, Liesbeth Coteur, E. Dilissen, P. Hellings, L. Dupont, J. Ceuppens. Respiratory Research, 2006
637 citations recorded by the source corpus.
BackgroundThe role of Th2 cells (producing interleukin (IL-)4, IL-5 and IL-13) in allergic asthma is well-defined. A distinct proinflammatory T cell lineage has recently been identified, called Th17 cells, producing IL-17A, a cytokine that induces CXCL8 (IL-8) and recruits neutrophils. Neutrophilic infiltration in the airways is prominent in severe asthma exacerbations and may contribute to airway gland hypersecretion, bronchial hyper-reactivity and airway wall remodelling in asthma.Aimto study the production of IL-17 in asthmatic airways at the mRNA level, and to correlate this with IL-8 mRNA, neutrophilic inflammation and asthma severity.MethodsWe obtained airway cells by sputum induction from healthy individuals (n = 15) and from asthmatic patients (n = 39). Neutrophils were counted on cytospins and IL-17A and IL-8 mRNA expression was quantified by real-time RT-PCR (n = 11 controls and 33 asthmatics).ResultsSputum IL-17A and IL-8 mRNA levels are significantly elevated in asthma patients compared to healthy controls. IL-17 mRNA levels are significantly correlated with CD3γ mRNA levels in asthmatic patients and mRNA levels of IL-17A and IL-8 correlated with each other and with sputum neutrophil counts. High sputum IL-8 and IL-17A mRNA levels were also found in moderate-to-severe (persistent) asthmatics on inhaled steroid treatment.ConclusionThe data suggest that Th17 cell infiltration in asthmatic airways links T cell activity with neutrophilic inflammation in asthma.
Subfields: Emergency Medical Services, Immunology, Physiology
View Paper: https://www.semanticscholar.org/paper/61499317ab780bb3fc35ee33544ee5a9ef53330a
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Citations in the corpus
- Treatment strategies for allergy and asthma (2008, background): “IL-17A is overexpressed in asthmatic airways in association with neutrophil influ”
- The cytokine network in asthma and chronic obstructive pulmonary disease. (2008, background): “Little is known about the role of Th17 cells in either asthma or COPD, but levels of IL-17A (the predominant product of Th17 cells) are increased in the sputum of individuals with asthma (41) and Th17 cells are increased in the airways of asthmatic subjects (42).”
- Immunology of asthma and chronic obstructive pulmonary disease (2008, background): “Little is known about the role of T H 17 cells in asthma or COPD, but increased concentrations of IL-17 (the predominant product of T H 17 cells) have been reported in the sputum of asthma patient”