Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts
By O. Beckonert, H. Keun, T. Ebbels, Jacob G. Bundy, Edward C. Holmes, John C. Lindon, Jeremy K. Nicholson. Nature Protocols, 2007
2,010 citations recorded by the source corpus.
Subfields: Molecular Biology, Nuclear and High Energy Physics, Radiology, Nuclear Medicine and Imaging
View Paper: https://www.semanticscholar.org/paper/c3953d23aef29a830eb205a01eaf341b897a5011
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In Molecular Biology
- David S. Wishart
- Jeremy K. Nicholson
- Ian A. Wilson
- Edward C. Holmes
- James Kinross
- J. Shockcor
- Cholsoon Jang
- Li Chen
In Nuclear and High Energy Physics
In Radiology, Nuclear Medicine and Imaging
Citations in the corpus
- The Microbiome and Butyrate Regulate Energy Metabolism and Autophagy in the Mammalian Colon (2011, neutral): “NMR Metabolomics Colonocytes were processed with the methanolchloroform-water method (Beckonert et al., 2007), and the polar phase was lyophilized, stored at 80 C, thawed and suspended in 550 ml of a 1.”
- Global metabolic profiling procedures for urine using UPLC–MS (2010, methodology): “The protocols for the efficient use of NMR spectroscopy in biofluids and tissues have been presented recentl”
- Somatic oxidative bioenergetics transitions into pluripotency-dependent glycolysis to facilitate nuclear reprogramming (2011, methodology): “For footprinting of extracellular metabolites (see the Supplemental Experimental Procedures), 540 ml of media collected following 24 hr of culture was added to 60 ml of D2O (Sigma) containing 5 mM sodium 3-(trimethylsilyl)propionate-2,2,3,3-d4 (TSP) (Sigma) for chemical shift reference and 81.84 mM ”
- MicroRNA-122 plays a critical role in liver homeostasis and hepatocarcinogenesis. (2012, neutral): “Lipid profiling by 1H-NMR spectroscopy (22) was performed to determine whether there were any differences in the lipid metabolite profile of the Mir122a–/– mice.”
- Fat, Fiber and Cancer Risk in African Americans and Rural Africans (2015, positive): “An NMR pulse sequence (recycle delay −90°-t 1 -90°-t m -90°-acquisition) and standard parameters were used to obtain standard one-dimensional 1 H NMR spectral data as described in Beckonert et al 67 .”
- Metabolomics and isotope tracing (2018, background): “This is feasible due to advances in metabolite measurement technologies like NMR and mass spectrometry (MS) (Fiehn, 2002; Beckonert et al., 2007).”
- A primer to nutritional metabolomics by NMR spectroscopy and chemometrics (2013, methodology): “, 2002) and in most cases only one analytical replicate is sufficient per time point (Beckonert et al., 2007).”
- Multivariate Analysis in Metabolomics. (2012, background): “High-resolution 1H NMR spectroscopy is especially suited for probing biofluids, cell lysates and tissues with almost no sample treatment and without bias [36, 37].”
- Metabolomics for Investigating Physiological and Pathophysiological Processes. (2019, methodology): “A large number of papers and reviews have been published on protocols or best practices for handling many different tissues, organs, or biofluids (34, 76, 292, 490, 508).”