Metabolomics and isotope tracing

By Cholsoon Jang, Li Chen, Joshua D. Rabinowitz. Cell, 2018

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Great strides have made over the past decade towards comprehensive study of metabolism. Mass spectrometry has played a central role, by enabling measurement of many metabolites simultaneously. Tracking metabolite labeling from stable isotope tracers can in addition reveal pathway activities. Here we describe the basics of metabolite measurement by mass spectrometry, including sample preparation, metabolomic analysis, and data interpretation. In addition, drawing on examples of successful experiments, we highlight the ways in which metabolomics and isotope tracing can illuminate biology.

Subfields: Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/de829e09c1047c64977a68251974433512b1e299