Beyond the sequence: cellular organization of genome function.

By Tom Misteli. Cell, 2007

1,185 citations recorded by the source corpus.

Genomes are more than linear sequences. In vivo they exist as elaborate physical structures, and their functional properties are strongly determined by their cellular organization. I discuss here the functional relevance of spatial and temporal genome organization at three hierarchical levels: the organization of nuclear processes, the higher-order organization of the chromatin fiber, and the spatial arrangement of genomes within the cell nucleus. Recent insights into the cell biology of genomes have overturned long-held dogmas and have led to new models for many essential cellular processes, including gene expression and genome stability.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/c238edcf281e4525d26c203bba6eaaf1a3a53499

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Citations in the corpus

Chromatin organization by an interplay of loop extrusion and compartmental segregation (2017, background): “Compartmental segregation of active and inactive chromatin is evident from microscopy (7, 38) and manifests as checkerboard pattern in Hi-C maps (2), but cannot be explained by loop extrusion.”
Architectural protein subclasses shape 3D organization of genomes during lineage commitment. (2013, positive): “Genomes are organized at multiple length scales into sophisticated higher-order architectures (Misteli, 2007).”
Nuclear architecture of rod photoreceptor cells adapts to vision in mammalian evolution. (2009, background): “In interphase nuclei, heterochromatin and euchromatin form distinct, spatially separated domains (Kosak et al., 2007; Misteli, 2007).”
Spatial coordination between cell and nuclear shape within micropatterned endothelial cells (2012, background): “, which is emerging as a potential regulator of genome functio”
Epigenetic control of T-helper-cell differentiation (2009, background): “Such 'chromatin looping' can bring distal regulatory elements in proximity to one another and to the promoters of their target genes, thereby facilitating gene expressio”
Long noncoding RNAs: Cellular address codes in development and disease (2013, neutral): “, and Misteli, T. (2010). Biogenesis of nuclear bodies.”
Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System (2013, positive): “CRISPR Imaging Allows Direct Visualization of Genetic Element Dynamics As demonstrated by our analysis ofMUC4 loci position distribution (Figure 6A), a straightforward application of CRISPR imaging is to monitor the position of specific genomic loci in the nucleus, which is an important mechanism fo”
Massive Genomic Rearrangement Acquired in a Single Catastrophic Event during Cancer Development (2011, background): “During interphase, chromosomes are relaxed with long loops of DNA winding through the nucleus: although given chromosomes occupy general nuclear territories, these tend to be loosely defined and nonexclusive (Misteli, 2007).”
Transgenerational Epigenetic Inheritance: Prevalence, Mechanisms, and Implications for the Study of Heredity and Evolution (2009, positive): “These reconstructions may be assisted by other chromatin components such as DNA methylation patterns (Martin and Zhang 2007), RNAs (Grewal and Jia 2007; Ringrose and Paro 2007), and by the location of the chromosomal domains within the nucleus (Misteli 2007).”