Conditional-Replication, Integration, Excision, and Retrieval Plasmid-Host Systems for Gene Structure-Function Studies of Bacteria

By A. Haldimann, B. Wanner. Journal of Bacteriology, 2001

617 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/bc1ad1e13b45db2c358b8921f17fd6b63b14a7fa

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In Genetics

1. Kim Lewis
2. Tobias Dörr
3. M. Vulić
4. Carol A. Gross
5. Ian B. Dodd
6. K. Shearwin
7. Rachel A. Schubert
8. J. Egan

In Molecular Biology

1. Kim Lewis
2. Tobias Dörr
3. M. Vulić
4. Carol A. Gross
5. Luke A. Gilbert
6. Jonathan S. Weissman
7. Max A. Horlbeck
8. Britt Adamson

Citations in the corpus

• Intracellular Detection and Evolution of Site-Specific Proteases Using a Genetic Selection System (2012, neutral): “conditional-replication, integration, and modular plasmid system [41].”
• A multiplexed single-cell CRISPR screening platform enables systematic dissection of the unfolded protein response (2016, methodology): “A uthor M anuscript A uthor M anuscript A uthor M anuscript A uthor M anuscript region variants were integrated into the dCas9- and mRFP-expressing strain at attL using the helper plasmid pINT-ts (Haldimann and Wanner, 2001), selecting for chloramphenicol resistance.”
• Ciprofloxacin Causes Persister Formation by Inducing the TisB toxin in Escherichia coli (2010, methodology): “For complementation experiments, the tisAB locus was cloned into the CRIM vector pCAH63 using primers RegiontisBAfwKpn1 (59-GTCGTCGGTACCTTGAGTATCGATCACAGTTTGCGT-39, Kpn1 site in bold) and RegiontisBArevKpn1 (59-GTCGTCGGTACCCCTTTGGTGCGACTTGAATCTG-39, Kpn1 site in bold) and inserted into the lambda at”
• Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection (2006, methodology): “Datsenko and Wanner, 2000 ) was made by PCR amplification of the Red recombinase genes from phage λ and cloning into pKD16, a derivative of INT-ts”
• Cro’s role in the CI–Cro bistable switch is critical for λ’s transition from lysogeny to lytic development (2007, methodology): “.b kan.imm434 was obtained by crossing the b kan insertion from pJWL464 onto .imm434 (Supplemental Material; Michalowski et al. 2004). b kan is referred to as WT, and all OR mutant phage carry the b kan insertion.”