Cro’s role in the CI–Cro bistable switch is critical for λ’s transition from lysogeny to lytic development
By Rachel A. Schubert, Ian B. Dodd, J. Egan, K. Shearwin. 2007
92 citations recorded by the source corpus.
CI represses cro; Cro represses cI. This double negative feedback loop is the core of the classical CI–Cro epigenetic switch of bacteriophage . Despite the classical status of this switch, the role in development of Cro repression of the PRM promoter for CI has remained unclear. To address this, we created binding site mutations that strongly impaired Cro repression of PRM with only minimal effects on CI regulation of PRM. These mutations had little impact on development after infection but strongly inhibited the transition from lysogeny to the lytic pathway. We demonstrate that following inactivation of CI by ultraviolet treatment of lysogens, repression of PRM by Cro is needed to prevent synthesis of new CI that would otherwise significantly impede lytic development. Thus a bistable CI–Cro circuit reinforces the commitment to a developmental transition.
Subfields: Ecology, Genetics, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/873dd6a8690bc3962b7873499c5ae8a3c46bfc07
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Citations in the corpus
- Network resilience (2020, background): “The lysogeny–lytic bistable switch in bacteriophage λ can be described by similar models [313, 314, 315, 316].”