The Mad1/Mad2 complex as a template for Mad2 activation in the spindle assembly checkpoint.

By Anna De Antoni, C. Pearson, D. Cimini, J. Canman, Valeria Sala, L. Nezi, Marina Mapelli, Lucia Sironi, M. Faretta, E. Salmon and 1 more. 2005

470 citations recorded by the source corpus.

Subfields: Cell Biology, Molecular Biology

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In Cell Biology

1. A. Musacchio
2. Hongtao Yu
3. Xuelian Luo
4. Stephen S. Taylor
5. J. Peters

In Molecular Biology

1. Hongtao Yu
2. Xuelian Luo
3. A. Musacchio
4. Stephen S. Taylor
5. J. Peters

Citations in the corpus

• Predicting multiple conformations via sequence clustering and AlphaFold2 (2023, neutral): “The closed state binds to CDC20 as part of MAD2’s function as a cell cycle checkpoint 26 .”
• Protein Metamorphosis: The Two-State Behavior of Mad2 (2008, methodology): “Kd,1 is estimated from dissociation constant of O-Mad2 binding to Mad1-bound C-Mad2 (De Antoni et al., 2005).”
• Mad2 overexpression promotes aneuploidy and tumorigenesis in mice. (2007, unknown): “This loading is thought to involve the association of a closed conformer of Mad2 (bound to Mad1 anchored at the kinetochore) with an open conformer capable of binding cdc20 and inhibiting APC activity (De Antoni et al., 2005).”
• The spindle assembly checkpoint. (2012, background): “reaction appears to be inhibited away from kinetochores, most likely via a mechanism that blocks the recruitment of O-Mad2 to the core complex [5,63,69].”
• The anaphase promoting complex/cyclosome: a machine designed to destroy (2006, background): “It has recently been proposed that this Mad1–Mad2 complex at kinetochores functions as a template for the assembly of Cdc20–Mad2 complexe”
• Riding the DUBway: regulation of protein trafficking by deubiquitylating enzymes (2006, unknown): “Transfected Nox4 also colocalizes with PTP1B in COS7 cells, and Nox4 enhances insulin receptor tyrosine phosphorylation through PTP1B inactivation , which is again consistent with spatial coordination between oxidant source and target (Mahadev et al., 2004; Martyn et al., 2006).”
• Bub3 reads phosphorylated MELT repeats to promote spindle assembly checkpoint signaling (2013, background): “For instance, forced retention of the Mad1-Mad2 ‘template’ complex (De Antoni et al., 2005) at kinetochores results in a protracted checkpointdependent arrest despite all chromosomes being bipolarly aligned at the metaphase plate (Gassmann et al., 2010; Maldonado and Kapoor, 2011), indicating that…”