Use of 16S rRNA and rpoB Genes as Molecular Markers for Microbial Ecology Studies

By Rebecca J. Case, Y. Boucher, I. Dahllöf, C. Holmström, W. Ford Doolittle, S. Kjelleberg. Applied and Environmental Microbiology, 2006

662 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/b8b551abb76a4ff14d4766fcbde838f4776b0ff0

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

In Molecular Biology

Citations in the corpus

The Variability of the 16S rRNA Gene in Bacterial Genomes and Its Consequences for Bacterial Community Analyses (2013, background): “or even better than that of the 16S rRNA [34–35].”
Revision of the Synechococcales (Cyanobacteria) through recognition of four families including Oculatellaceae fam. nov. and Trichocoleaceae fam. nov. and six new genera containing 14 species (2018, background): “…coding genes, for example the rpoB and rpoC1 loci of the rpo gene family encoding for different beta subunits of DNa-dependent rNa polymerase (Case et al. 2007, Gaget et al. 2011, fergusson & Saint 2000, Wilson et al. 2000); rbcl, a gene encoding the large subunit of ruBisCo, a critical…”
A Primer on Metagenomics (2010, background): “1 [66], but in a recent publication it has been shown that 16S rDNA gene copy numbers may vary between 1 and 15 [67,68].”
Molecular tools in rhizosphere microbiology—from single-cell to whole-community analysis (2009, background): “In addition, the rpoB gene has thus been shown to contain enough phylogenetic information for diversity studies (Mollet et al. 1997; Case et al. 2007).”
Structure and Function of the Bacterial Root Microbiota in Wild and Domesticated Barley (2015, background): “These quantitative differences can be at least partially attributed to the fact that Beta- and Gammaproteobacteria possess multiple ribosomal RNA operon copies (Case et al., 2007).”
Insights from 20 years of bacterial genome sequencing (2015, background): “It is well known, however, that very similar 16S rRNA gene sequences can lead to poor resolution at the species level (Case et al. 2007).”
Incorporating 16S Gene Copy Number Information Improves Estimates of Microbial Diversity and Abundance (2012, methodology): “The use of a single-copy protein coding gene such as rpoB as a microbial barcode would avoid this problem [11,19,20],”