Structure and Function of the Bacterial Root Microbiota in Wild and Domesticated Barley

By D. Bulgarelli, Ruben Garrido-Oter, Philipp C. Münch, A. Weiman, Johannes Dröge, Yao Pan, Alice Carolyn McHardy, Paul Schulze-Lefert. Cell Host and Microbe, 2015

1,160 citations recorded by the source corpus.

Summary The microbial communities inhabiting the root interior of healthy plants, as well as the rhizosphere, which consists of soil particles firmly attached to roots, engage in symbiotic associations with their host. To investigate the structural and functional diversification among these communities, we employed a combination of 16S rRNA gene profiling and shotgun metagenome analysis of the microbiota associated with wild and domesticated accessions of barley (Hordeum vulgare). Bacterial families Comamonadaceae, Flavobacteriaceae, and Rhizobiaceae dominate the barley root-enriched microbiota. Host genotype has a small, but significant, effect on the diversity of root-associated bacterial communities, possibly representing a footprint of barley domestication. Traits related to pathogenesis, secretion, phage interactions, and nutrient mobilization are enriched in the barley root-associated microbiota. Strikingly, protein families assigned to these same traits showed evidence of positive selection. Our results indicate that the combined action of microbe-microbe and host-microbe interactions drives microbiota differentiation at the root-soil interface.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/805e4e1bc6de64d7d2eab4e1d5999ce53174a524

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Citations in the corpus

A review on the plant microbiome: Ecology, functions, and emerging trends in microbial application (2019, background): “[44] investigated the root microbiota of different barley accessions and found that the host innate immune system and root metabolites mainly shaped root microbial community structure.”
Feed Your Friends: Do Plant Exudates Shape the Root Microbiome? (2018, background): “Initial evidence for an influence of plant genotype on rhizobiome composition was that similar rhizobiomes assembled in association with arabidopsis (Arabidopsis thaliana) and barley (Hordeum vulgare) grown in the same experimental conditions, although they displayed different relative abundances an”
Understanding and exploiting plant beneficial microbes (2017, background): “However, metagenomic datasets from different rhizospheres exhibit little overlap in plant-enriched functions [13,15].”
The Role of Soil Microorganisms in Plant Mineral Nutrition—Current Knowledge and Future Directions (2017, background): “When comparing accessions or varieties of the same species, genotypic effects on microbiome structure have been seen amongst Arabidopsis, maize, and barley (Bulgarelli et al., 2012, 2015; Peiffer et al., 2013).”
Plant compartment and biogeography affect microbiome composition in cultivated and native Agave species (2015, background): “Interestingly, prokaryotic communities in the desert environments surveyed here do not differ markedly at the phylum level from other soil environments described previously in other phytobiome studies (Lundberg et al., 2012; Bulgarelli et al., 2015; Edwards et al., 2015; Zarraonaindia et al., 2015).”
Impact of plant domestication on rhizosphere microbiome assembly and functions (2015, background): “Similarly, in a study with different barley genotypes, Bulgarelli et al. (2015) found that the host genotype accounts for approximately 5.7 % of the variance in the rhizosphere microbiome composition.”