Characterization of antibacterial activity from protein hydrolysates of the macroalga Saccharina longicruris and identification of peptides implied in bioactivity
By L. Beaulieu, S. Bondu, K. Doiron, Laurie-Eve Rioux, S. Turgeon. 2015
104 citations recorded by the source corpus.
Abstract The macroalga Saccharina longicruris proteins were extracted and hydrolysed with the enzyme trypsin in order to recover antibacterial peptides, which could be used in reducing food spoilage. The >10 kDa protein hydrolysate fraction exhibited activity against the bacterium Staphylococcus aureus with a significant decrease of the maximum specific growth rate, µmax, from concentrations of 0.31 mg/mL to 2.5 mg/mL. Protein precursors were identified in a subfraction (Fr1) enriched in antibacterial peptides using liquid chromatography–tandem mass spectrometry (LC–MS/MS) with database searches. Identified peptides came from different precursors such as a protein similar to ubiquitin, histones, a hypothetical leucine rich repeat protein and a ribosomal protein. These proteins might be associated with the innate immune defences in S. longicruris. Peptidic sequences were chemically synthesized and their bioactivity confirmed, suggesting that activity probably results from synergies between peptides. This is the first time that antibacterial peptides originating from algal protein hydrolysates were isolated and characterized.
Subfields: Aquatic Science, Insect Science, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/b80dba8bb6b53a7edbcddc05ec20a10139bfb4a0
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In Aquatic Science
In Molecular Biology
Citations in the corpus
- Algal Proteins: Extraction, Application, and Challenges Concerning Production (2017, unknown): “Antimicrobial peptides have been identified from Saccharina longicruris protein hydrolysates, significantly decreasing the growth rate of Staphylococcus aureus [199].”
- The development of seaweed-derived bioactive compounds for use as prebiotics and nutraceuticals using enzyme technologies (2017, background): “…Fraction >10 kDa (45.4% w/w) ↑ Antibacterial activity against Staphylococcus aureus ↓ Maximum specific growth rate at 2.5 mg/mL from 0.19 to 0.02 µmax/h Beaulieu et al. (2015) Pyropia columbina (R) (Protein extracts) - Alkaline protease (Danisco) - Flavourzyme (Sigma) Simple hydrolysis (A)…”
- Seaweeds: A traditional ingredients for new gastronomic sensation (2017, background): “Beaulieu, Bondu, Doiron, Rioux, and Turgeon (2015) have extracted antibacterial peptides from the brown seaweed Saccharina longicruris and the identi fi ed peptides were associated to proteins precursors similar to ubiquitin, leucine rich repeat protein, histone, and a ribosomal structure, which are…”