The uncultured microbial majority.

By M. Rappé, S. Giovannoni. Annual Review of Microbiology, 2003

2,058 citations recorded by the source corpus.

Subfields: Ecology, Environmental Chemistry, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/b762aceb7d94a73637fb761f9168fc8dc3601bf6

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Citations in the corpus

Changes in bacterial activity and community structure in response to dissolved organic matter in the Hudson River, New York (2004, background): “…factors include inorganic nutrients such as phosphate and iron (Pakulski et al. 1996, Cotner et al. 1997), grazing (2imek et al. 1997, Suzuki 1999, Riemann et al. 2000, Pernthaler et al. 2001, Beardsley et al. 2003, Cottrell & Kirchman 2004), viral lysis (Fuhrman 2000), temperature (Pomeroy &…”
Interactions between microbial consortia in biofilms: a paradigm shift in rumen microbial ecology and enteric methane mitigation (2014, background): “Application of technologies that were independent of cultured microbes has shown that species diversity in the rumen has been vastly underestimated (Rappé and Giovannoni 2003; Edwards et al. 2008).”
Microbial Biodiversity in Groundwater Ecosystems (2009, background): “Designations for uncultured lineages follow Rheims et al. (1996), Dojka et al.”
Use of Ichip for High-Throughput In Situ Cultivation of “Uncultivable” Microbial Species (2010, background): “However, by and large the gap between microbial diversity in nature and that in culture collections remains unchanged, and most microbial phyla still have no cultivable representatives (25, 29).”
UniFrac: a New Phylogenetic Method for Comparing Microbial Communities (2005, background): “] Only half of the 52 major bacterial lineages described in the last comprehensive review have cultivated representatives, and widespread, numerically dominant phylotypes are often only distantly related to culturable strains (37).”
Microbial macroecology: highly structured prokaryotic soil assemblages in a tropical deciduous forest (2005, background): “Nevertheless, only 26 of the more than 50 major lineages (Phyla) of the domain Bacteria are represented in cultivated strains (Rappé & Giovannoni, 2003), and there are only about 4500 species that have been characterized.”
At Least 1 in 20 16S rRNA Sequence Records Currently Held in Public Repositories Is Estimated To Contain Substantial Anomalies (2005, background): “At least half of the 53 phyla named in 2003 are currently known only from 16S rRNA gene sequences amplified from the environment by PCR (15), and this number is growing (4).”
The Rebirth of Culture in Microbiology through the Example of Culturomics To Study Human Gut Microbiota (2015, background): “Among these species, more than half of the known phyla have not yet been isolated in culture (127, 128).”
Metagenomics: genomic analysis of microbial communities. (2004, background): “∗ Indicates phyla that Hugenholtz et al. (54) or Rappé & Giovannoni (90) noted as particularly abundant in environmental samples but underrepresented in culture.”
Accurate taxonomy assignments from 16S rRNA sequences produced by highly parallel pyrosequencers (2008, background): “Because the vast majority of microbes have not been cultured, and perhaps cannot be cultured using existing techniques (2), culture-independent techniques that analyze small subunit ribosomal RNA sequences (16S rRNA in the case of bacteria and archaea), amplified by PCR directly from environmental s”
Ribosomal Multi-Locus Sequence Typing: universal characterisation of bacteria from domain to strain (2012, background): “…of a unified scheme has, however, been hindered by the extensive diversity of the bacterial domain with members, most yet to be cultured (Rappé & Giovannoni, 2003), found in virtually all known biological http://mic.sgmjournals.org 1009 niches and participating in the majority of…”