Use of Ichip for High-Throughput In Situ Cultivation of “Uncultivable” Microbial Species

By Dominica Nichols, N. Cahoon, E. Trakhtenberg, L. Pham, A. Mehta, A. Bélanger, T. Kanigan, Kim Lewis, S. Epstein. Applied and Environmental Microbiology, 2010

712 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Biophysics, Ecology

View Paper: https://www.semanticscholar.org/paper/413785747afb05eaf7ec8871174ce83bae3e6410

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biomedical Engineering

1. An-Ping Zeng

In Ecology

1. A. Goodman
2. Yang Bai
3. Jeffrey I. Gordon
4. Gautam Dantas
5. Jeremiah J. Faith
6. K. Narisawa
7. K. Peay
8. Q. Shen

Citations in the corpus

• Extensive personal human gut microbiota culture collections characterized and manipulated in gnotobiotic mice (2011, background): “, sterile rumen or human fecal extracts) and other approaches for more closely approximating a native gut habitat, undoubtedly will allow additional members of the human gut microbiota to be cultured in vitro (10, 11).”
• The Rebirth of Culture in Microbiology through the Example of Culturomics To Study Human Gut Microbiota (2015, methodology): “In addition, the species isolated by using the ichip method had significant phylogenetic novelty (143).”
• Endolichenic fungi: a new source of rich bioactive secondary metabolites on the horizon (2016, background): “Multiple reviews have highlighted the metabolite diversity and potential of endophytic fungi to produce pharmaceutically valuable natural products (Kaul et al. 2012; Nisa et al. 2015; Proksch et al. 2010; Strobel et al. 2004; Tan and Zou 2001).”
• Biosystems analysis and engineering of microbial consortia for industrial biotechnology (2010, background): “[55] reported a microfluidic microsystem approach as a high-throughput screening method for uncultivable organisms.”
• Specialized metabolic functions of keystone taxa sustain soil microbiome stability (2021, background): “Novel approaches such as the isolation chip [26] and microbial trap [27] have been developed to study such taxa.”
• Core microbiomes for sustainable agroecosystems (2018, background): “Such microfluidic devices 91 for culturing microorganisms give hope to the possibility of adding novel microbial species/strains to culture collections.”