RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions

By Wiedenheft, Van Duijn. 2011

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1. Jennifer A. Doudna
2. Rodolphe Barrangou
3. Ambarish Biswas
4. S. H. Sternberg
5. Ryan T. Leenay
6. Devaki Bhaya
7. S. Gratz
8. Kenneth R. Maksimchuk

Citations in the corpus

• Identifying and visualizing functional PAM diversity across CRISPR-Cas systems (2016, background): “, 2015) or the other type I subtypes that form a stable Cascade-like complex (Brendel et al., 2014; Wiedenheft et al., 2011), with the potential to identify”
• CRISPRTarget (2013, background): “15,20,39 For type III systems, it is unclear if a seed sequence exists and no PAMs have been identified.”
• CRISPR-Cas systems: prokaryotes upgrade to adaptive immunity (2014, background): “…complex in type I systems is called CRISPR-associated complex for antiviral defense (Cascade) (Brouns et al., 2008), which is formed by Cse1, Cse2, Cas7, Cas5, and Cas6e subunits in E. coli (type I-E) and Csy1, Csy2, Csy3, and Cas6f in Pseudomonas aeruginosa (type I-F) (Wiedenheft et al., 2011).”
• CRISPRDetect: A flexible algorithm to define CRISPR arrays (2016, background): “In Type I, Type II and Type V systems, the bases at one end of the spacer are usually part of a ‘seed’ sequence, that is critical for base-pairing, target recognition and interference [36–40].”
• CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation. (2011, background): “The process is initiated at the seed sequence adjacent to the PAM and then propagated along the protospacer region in a 5′→3′ direction over the complete protospacer region (97, 119).”
• RNA-guided genetic silencing systems in bacteria and archaea (2012, background): “Each crRNAs is packaged into a large surveillance complex that patrols the intracellular environment and mediates the detection and destruction of foreign nucleic acid target”
• CRISPR-Cas Systems: RNA-mediated Adaptive Immunity in Bacteria and Archaea (2013, background): “…of type I targeting complexes Jore et al. Wiedenheft et al. 2011 CryoEM structure determination of type I targeting complex (Cascade) Jore et al. Wiedenheft et al. 2011 Characterisation of the type II tracrRNA-based processing mechanism Delcheva et al. 2011 Defining limits of type I DNA…”
• Highly Specific and Efficient CRISPR/Cas9-Catalyzed Homology-Directed Repair in Drosophila (2014, background): “…strategies consider both the number and the location of mismatches to evaluate all potential off-target cleavage sites (Semenova et al. 2011; Wiedenheft et al. 2011; Cradick et al. 2013; Fu et al. 2013; Hsu et al. 2013; Mali et al. 2013b; Pattanayak et al. 2013; Yang et al. 2013; Cho et…”