RNA-guided genetic silencing systems in bacteria and archaea

By B. Wiedenheft, S. H. Sternberg, Jennifer A. Doudna. Nature, 2012

1,788 citations recorded by the source corpus.

Subfields: Ecology, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/e9a6e6759052074012abe3df66ec59c9001617f7

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

1. Jennifer A. Doudna
2. Rodolphe Barrangou
3. L. Marraffini
4. E. Charpentier
5. Philippe Horvath
6. G. Gasiunas
7. V. Šikšnys
8. Lei S. Qi

In Genetics

1. Jennifer A. Doudna
2. E. Charpentier
3. Rodolphe Barrangou
4. L. Marraffini
5. Lei S. Qi
6. Mark A. Tester
7. S. Negrão
8. S. Roy

In Molecular Biology

1. Jennifer A. Doudna
2. Feng Zhang
3. Lei S. Qi
4. Rodolphe Barrangou
5. J. Keith Joung
6. E. Charpentier
7. L. Marraffini
8. P. Hsu

Citations in the corpus

• CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes (2013, background): “Prokaryotes have evolved diverse RNA-mediated systems that use short CRISPR RNAs (crRNAs) and Cas (CRISPR-associated) proteins to detect and defend against invading DNA elements (Bhaya et al., 2011; Marraffini and Sontheimer, 2008, 2010; Wiedenheft et al., 2012).”
• CRISPRTarget (2013, background): “5,44 RNAi utilizes miRNAs of ~20–22 bases that recognize specific mRNA targets.”
• Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis (2013, background): “All of these types and subtypes use short CRISPR RNAs (crRNAs) (16, 17) to specify genetic interference via the destruction of invading nucleic acids (18).”
• One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering (2013, neutral): “Bacteria and archaea have evolved an RNA-based adaptive immune system that uses CRISPR (clustered regularly interspaced short palindromic repeat) and Cas (CRISPR-associated) proteins to detect and destroy invading viruses and plasmids (Horvath and Barrangou, 2010; Wiedenheft et al., 2012).”
• CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool (2015, background): “For targeted genome editing the c lustered r egularly i nterspaced s hort p alindromic r epeats (CRISPR)/ CRISPR associated 9 (Cas9) system, initially discovered as ‘ immune response ’ in archaea and bacteria, has rapidly evolved as the tool of choice [10 – 12].”