Channelrhodopsin-2 and optical control of excitable cells

By Feng Zhang, Liping Wang, Ed Boyden, Karl Deisseroth. Nature Methods, 2006

729 citations recorded by the source corpus.

Subfields: Cellular and Molecular Neuroscience, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/b5d2fa43ebf1773968128f10c24d1e5e58a1c15c

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cellular and Molecular Neuroscience

1. Karl Deisseroth
2. Lief Ericsson Fenno
3. Ofer Yizhar
4. Mark T. Harnett
5. Karel Svoboda
6. Feng Zhang
7. S. Tonegawa
8. Liping Wang

In Molecular Biology

1. Karl Deisseroth
2. Mark T. Harnett
3. Lief Ericsson Fenno
4. Feng Zhang
5. G. Feng
6. Karel Svoboda
7. J. Wells
8. S. Tonegawa

Citations in the corpus

• The Development and Application of Optogenetics (2011, background): “Building both on our finding that adequate retinal is present within mammalian brain tissue (Deisseroth et al. 2006, Zhang et al. 2006) and on a long history of elegant GPCR structure-function work from Khorana, Kobilka, Caron, Lefkowitz, and others, we have determined that GPCRs can be converted…”
• Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures (2010, background): “In particular, we have found that optical manipulation of activity in neural circuits with light-sensitive rhodopsins, such as the Chlamydomonas channelrhodopsin-2 (ChR2”
• Cell type-specific genetic and optogenetic tools reveal novel hippocampal CA2 circuits (2013, positive): “For this purpose, we infected DGGC-specific Cre mice with a Cre-dependent adeno-associated virus carrying channelrhodopsin2-YFP 29 (AAV9-EF1 α -DIO-ChR2-YFP) to allow for DGGC-specific expression of ChR2-YFP (Fig.”
• An optical neural interface: in vivo control of rodent motor cortex with integrated fiberoptic and optogenetic technology (2007, background): “with previous observations of mCherry-ChR2 expression [22].”
• Channelrhodopsin-2–assisted circuit mapping of long-range callosal projections (2007, background): “Notably, addition of retinal to the extracellular medium was not necessary, indicating that the level of endogenous retinal in a brain slice is sufficient to make ChR2 functiona”
• Multimodal fast optical interrogation of neural circuitry (2007, background): “Author Information The GenBank accession number is EF474018 for the ‘mammalianized’ NpHR sequence and EF474017 for the ‘mammalianized’ ChR2(1-315) sequence.”
• Sexually dimorphic neurons in the ventromedial hypothalamus govern mating in both sexes and aggression in males (2013, methodology): “This transgene was inserted in reverse orientation into the plasmid pAAV-EF1a-DIO-hChR2(H134R)-EYFP-WPRE-pA such that it replaced hChR2(H134R)-EYFP (Zhang et al., 2006).”
• Nictation, a dispersal behavior of the nematode Caenorhabditis elegans, is regulated by IL2 neurons (2011, background): “Optogenetic activation of IL2 neurons initiates nictation To test whether the activation of IL2 neurons regardless of the presence of the mechanosensory function of the neurons can increase the probability of nictation, we optogenetically activated IL2 in either wild-type or daf-2(e1370); daf-10”
• Burst timing-dependent plasticity of NMDA receptor-mediated transmission in midbrain dopamine neurons: A putative cellular substrate for reward learning (2009, positive): “selectively targeted the light-activated gene channelrhodopsin-2 (ChR2) (Arenkiel et al., 2007; Boyden et al., 2005; Zhang et al., 2006; Zhang et al., 2007) to DA neurons via the tyrosine hydroxylase gene in Cre transgenic animals with local injection of adenoassociated virus.”