A Chemical Platform for Improved Induction of Human iPS Cells

By Tongxiang Lin, R. Ambasudhan, Xu Yuan, Wen-lin Li, S. Hilcove, Ramzey Abujarour, Xiangyi Lin, H. S. Hahm, E. Hao, A. Hayek and 1 more. Nature Methods, 2009

608 citations recorded by the source corpus.

The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells.

Subfields: Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/b3fca667fdc0b3a29c39f0c03678187b0c28daf6

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Citations in the corpus

Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells (2011, background): “Inhibitors to the type I TGF-β receptor (TβRI) kinase have been shown to enhance reprogramming to mouse and human iPSC”
A mesenchymal-to-epithelial transition initiates and is required for the nuclear reprogramming of mouse fibroblasts. (2010, background): “, 2009; Maherali and Hochedlinger, 2009) and human (Lin et al., 2009) iPSC generation.”
Reprogramming of human primary somatic cells by OCT4 and chemical compounds. (2010, methodology): “We first attempted to further optimize the reprogramming process using four or three TFs (i.e., OSKM or OSK) in neonatal human epidermal keratinocytes (NHEKs) by testing various inhibitors of TGFb and MAPK pathways at different concentrations using previously reported human iPSC characterization met”
Induced pluripotency: history, mechanisms, and applications. (2010, background): “This could occur by facilitating the binding of Oct4 and Sox2 to target genes; for example, by establishing or maintaining activating histone methylation (CH Lin et al. 2009) and acetylation (Knoepfler 2008) marks.”
Senescence impairs successful reprogramming to pluripotent stem cells. (2009, background): “The same was observed in human cells however in this case in conjunction with inhibition of mitotic activated protein kinase (MAPK) pathway (Lin et al., 2009).”