Real-Time DNA Sequencing from Single Polymerase Molecules

By J. Eid, Adrian N. Fehr, J. Gray, K. Luong, J. Lyle, Geoff A. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman and 44 more. Science, 2009

2,277 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/b0588d6e0753e7e3f82c4aa4f1609764179b12de

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biomedical Engineering

1. Mihai Pop
2. Mitchell J. Machiela
3. Stephen J. Chanock

In Molecular Biology

1. Heng Li
2. Steven L. Salzberg
3. Sergey Koren
4. Adam M. Phillippy
5. Michael C. Schatz
6. Mihai Pop
7. Richard Durbin
8. Brian Walenz

Citations in the corpus

• Current challenges and best-practice protocols for microbiome analysis (2019, background): “Another huge advantage of PacBio is its ability to produce much longer reads ranging between 10 and 50 kbp with an average read accuracy of ∼85% [140].”
• The Third Revolution in Sequencing Technology. (2018, background): “The first ‘true’ TGS technology was released on the market in 2011 by Pacific Biosciences (PacBio) and is termed ‘single-molecule real-time’ (SMRT) sequencing [13].”
• Canu: scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation (2016, background): “However, in contrast to improved read length, single-molecule sequencing is less accurate than past technologies (Eid et al. 2009; Schneider and Dekker 2012), requiring sensitive alignment methods and limiting the discrimination of divergent alleles and non-exact repeats.”
• Microbial Biodiversity and Bioremediation Assessment Through Omics Approaches (2020, methodology): “Pacific Biosciences developed the first genomic sequencer using single-molecule real-time technology (SMRT) approach (Eid et al., 2009; Meena et al., 2017d).”
• Integrating Hi-C links with assembly graphs for chromosome-scale assembly (2018, methodology): “A draft assembly is generated from long reads such as Pacific Biosciences (Eid et al., 2009) or Oxford Nanopore (Jain et al., 2016).”
• Computational methods for discovering structural variation with next-generation sequencing (2009, background): “Strengths, limitations, and the road ahead Despite current limitations, emerging technologie”
• Detection and quantification of rare mutations with massively parallel sequencing (2011, background): “describe another powerful strategy for reducing errors (51).”
• Merqury: reference-free quality, completeness, and phasing assessment for genome assemblies (2020, background): “With recent advances in long-read [1 – 3] and long-range sequencing technologies [4 – 6], new assembly pipelines are generating more continuous, complete, and accurate diploid genome assemblies than ever before [4, 7 – 14].”
• Assembly of long error-prone reads using de Bruijn graphs (2016, methodology): “When the first reads generated using Single Molecule Real Time (SMRT) sequencing technology were made available [18], most researchers were skeptical about the ability of existing algorithms to generate highquality assemblies from error-prone SMRT reads.”
• RNA Sequencing and Analysis (2015, positive): “Single-molecule-based platforms such as PacBio enable single-molecule real-time (SMRT) sequencing (Eid et al. 2009).”