The Third Revolution in Sequencing Technology.

By E. van Dijk, Y. Jaszczyszyn, Delphine Naquin, C. Thermes. Trends in Genetics, 2018

916 citations recorded by the source corpus.

Forty years ago the advent of Sanger sequencing was revolutionary as it allowed complete genome sequences to be deciphered for the first time. A second revolution came when next-generation sequencing (NGS) technologies appeared, which made genome sequencing much cheaper and faster. However, NGS methods have several drawbacks and pitfalls, most notably their short reads. Recently, third-generation/long-read methods appeared, which can produce genome assemblies of unprecedented quality. Moreover, these technologies can directly detect epigenetic modifications on native DNA and allow whole-transcript sequencing without the need for assembly. This marks the third revolution in sequencing technology. Here we review and compare the various long-read methods. We discuss their applications and their respective strengths and weaknesses and provide future perspectives.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/c90b2e30af495aa02fc8d15c1b8272173348b1e1

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In Molecular Biology

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Citations in the corpus

• Comparing bioinformatic pipelines for microbial 16S rRNA amplicon sequencing (2020, background): “Rapid progress in DNA sequencing technology has provided ever-increasing outputs coupled with lowered costs, facilitating an explosion in amplicon sequencing studies [3].”