Detection and quantification of rare mutations with massively parallel sequencing

By I. Kinde, Jian Wu, N. Papadopoulos, Kenneth W. Kinzler, Bert Vogelstein. Proceedings of the National Academy of Sciences of the United States of America, 2011

1,138 citations recorded by the source corpus.

Subfields: Cancer Research, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/48b77e18d9743c5fcadac2338c6e43f783c2bd04

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cancer Research

1. A. Bardelli
2. L. Diaz
3. Lawrence Loeb
4. J. Salk
5. Michael W. Schmitt
6. Bert Vogelstein
7. Victor E. Velculescu
8. Kenneth W. Kinzler

In Genetics

1. J. Reis-Filho
2. Mitchell Dowsett
3. Bert Vogelstein
4. N. Turner
5. James Robert White
6. C. Tomasetti
7. N. Rosenfeld
8. P. Park

In Molecular Biology

1. L. Diaz
2. A. Bardelli
3. Victor E. Velculescu
4. Joshua Quick
5. Bert Vogelstein
6. Kenneth W. Kinzler
7. J. Reis-Filho
8. Carlos Caldas

Citations in the corpus

• Direct detection of early-stage cancers using circulating tumor DNA (2017, background): “(28) reported the use of a large number of random exogenous barcodes as unique identifiers for analysis of rare mutations in DNA populations.”
• VDJtools: Unifying Post-analysis of T Cell Receptor Repertoires (2015, methodology): “To remove quantitative biases and reduce possible impact from PCR and sequencing artifacts, we have utilized unique molecular identifiers [10,45,46].”
• Noninvasive Identification and Monitoring of Cancer Mutations by Targeted Deep Sequencing of Plasma DNA (2012, methodology): “Such approaches have recently been complemented by methods for examination of individual amplicons at great depth (30).”
• Enhancing the accuracy of next-generation sequencing for detecting rare and subclonal mutations (2018, methodology): “Molecular barcodes come in two forms: exogenous and endogenous 53,55 .”
• Mutation tracking in circulating tumor DNA predicts relapse in early breast cancer (2015, methodology): “org 26 though techniques such as barcoding for error correction (20) may allow MPS to challenge dPCR for sensitivity.”
• Detection of ultra-rare mutations by next-generation sequencing (2012, methodology): “The most sensitive approach previously reported for improving accuracy of next-generation sequencing involves use of a random tag sequence in a PCR primer (23).”
• An amplicon-based sequencing framework for accurately measuring intrahost virus diversity using PrimalSeq and iVar (2018, methodology): “utilization of different molecular approaches, including the 1D(2) method (where template and complementary strands of each fragment are sequenced) [40], tandem repeat consensus techniques [36, 51] or unique molecular identifiers [52].”