Circulating Skeletal Stem Cells

By S. Kuznetsov, M. Mankani, S. Gronthos, K. Satomura, P. Bianco, P. Robey. Journal of Cell Biology, 2001

745 citations recorded by the source corpus.

We report the isolation of adherent, clonogenic, fibroblast-like cells with osteogenic and adipogenic potential from the blood of four mammalian species. These cells phenotypically resemble but are distinguishable from skeletal stem cells found in bone marrow (stromal stem cells, “mesenchymal stem cells”). The osteogenic potential of the blood-borne cells was proven by an in vivo transplantation assay in which either polyclonal or single colony–derived strains were transplanted into the subcutis of immunocompromised mice, and the donor origin of the fully differentiated bone cells was proven using species-specific probes. This is the first definitive proof of the existence of circulating skeletal stem cells in mammals.

Subfields: Epidemiology, Genetics, Oncology

View Paper: https://www.semanticscholar.org/paper/afc5ce7f18b572c704c0467473d490a970f78f1b

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Genetics

1. Rocky S. Tuan
2. D. Baksh
3. L. Song
4. P. Robey
5. P. Bianco
6. S. Gronthos
7. S. Shi
8. Elizabeth Cho

In Oncology

1. E. Eriksen
2. I. J. Fidler
3. Rocky S. Tuan
4. R. Weinberg
5. J. Massagué
6. Scott Valastyan

Citations in the corpus

• Cellular mechanisms of bone remodeling (2010, background): “It is still debatable whether all cells involved in remodeling arrive via the circulation, but while circulating osteoclast precursors were demonstrated more than a decade ago, there is now increasing evidence that osteoblast lineage cells are also present in the circulation strengthening the involv”
• Carcinoma-associated fibroblast-like differentiation of human mesenchymal stem cells. (2008, background): “They are present in large numbers in human blood for the first 12 weeks of gestation and circulating MSCs, albeit in low numbers, exist in the adult (16, 17).”
• Stem cells in tissue engineering (2001, background): “, and cells able to regenerate bone are also found in bloo”
• Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy (2004, methodology): “[ 100 ] BM aspirates 34.2 – 6 in 1 x 105 • Adherent colonies of fibroblastic-like cells”
• Mesenchymal stem cell homing: the devil is in the details. (2009, neutral): “Studies that support the presence of MSCs within blood have reported only minute quantities of circulating MSCs (Kuznetsov et al., 2001), whereas several studies report the inability to locate any circulating MSCs at all (reviewed in He et al., 2007).”
• Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood. (2005, background): “Human MSC have been isolated from adipose tissue [8], umbilical cord blood [9–12], peripheral blood [13,14], connective tissues of the dermis, and skeletal muscle [15].”
• Bone marrow-derived mesenchymal stem cells as vehicles for interferon-beta delivery into tumors. (2002, background): “It remains unclear whether bone marrow cells developing into MSCs under in vitro conditions are identical to circulating mesenchymal precursors in the peripheral blood (19).”
• Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF. (2001, methodology): “Furthermore, the levels of type II collagen and type, X £^ for :# inv FGF(l) MSC from the 9th passage cultures than in FGF(2)MSC from the 9th passage cultures (Fig.”