Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF.
By S. Tsutsumi, A. Shimazu, K. Miyazaki, Haiou Pan, C. Koike, E. Yoshida, K. Takagishi, Y. Kato. Biochemical and Biophysical Research Communications - BBRC, 2001
611 citations recorded by the source corpus.
Mesenchymal stem cells (MSC) that can differentiate to various connective tissue cells may be useful for autologous cell transplantation to defects of bone, cartilage, and tendon, if MSC can be expanded in vitro. However, a short life span of MSC and a reduction in their differentiation potential in culture have limited their clinical application. The purpose of this study is to identify a growth factor(s) involved in self-renewal of MSC and the maintenance of their multilineage differentiation potential. Fibroblast growth factor-2 (FGF-2) markedly increased the growth rate and the life span of rabbit, canine, and human bone marrow MSC in monolayer cultures. This effect of FGF-2 was more prominent in low-density cultures than in high-density cultures. In addition, all MSC expanded in vitro with FGF-2, but not without FGF-2, differentiated to chondrocytes in pellet cultures. The FGF+ MSC also retained the osteogenic and adipogenic potential throughout many mitotic divisions. These findings suggest that FGFs play a crucial role in self-renewal of MSC.
Subfields: Genetics, Surgery, Urology
View Paper: https://www.semanticscholar.org/paper/62b19e0a946090f13230ca5a8914e89ac863566e
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Citations in the corpus
- Adult mesenchymal stem cells and cell-based tissue engineering (2002, result): “Other studies, however, have shown the differential potential of human MSCs to be unaffected by fibroblast growth factor-2 treatment, notwithstanding the proliferative effects [57].”
- Platelet lysates promote mesenchymal stem cell expansion: A safety substitute for animal serum in cell‐based therapy applications (2005, background): “…IGF-1, and PDGFs are key factors in supporting MSC ex vivo expansion and maintaining their multilineage potential for further differentiation (Gibbons et al., 1995; Cassiede et al., 1996; Hanada et al., 1997; Banfi et al., 2000; Muraglia et al., 2000; Tsutsumi et al., 2001; Walsh et al., 2003).”
- Mesenchymal stromal cells. Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation (2007, positive): “FGF2 maintains the stem state of MSCs from a variety of species by prolonging their viability in culture [16], sometimes in a cell-autonomous fashion [17].”