The utility of Ki-67 and BrdU as proliferative markers of adult neurogenesis.

By N. Kee, S. Sivalingam, R. Boonstra, J. Martin Wojtowicz. Journal of Neuroscience Methods, 2002

817 citations recorded by the source corpus.

Subfields: Cellular and Molecular Neuroscience, Developmental Neuroscience, Genetics

View Paper: https://www.semanticscholar.org/paper/ad280b6eccd18e4630244af7a7cc2b62cd8eb15b

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Citations in the corpus

Antidepressants increase neural progenitor cells in the human hippocampus (2009, background): “Dividing cells become neurons in the granule cell layer (GCL) of the DG in the adult human hippocampus where BrdU immunoreactive (-IR) cells double-label with antibodies specific to neurons (neuron-specific enolase, calbindin, and neuron-specific nuclear protein (NeuN, Eriksson et al, 1998).”
Short‐term and long‐term survival of new neurons in the rat dentate gyrus (2003, background): “%) of the BrdU-labeled cells in the gcl expressed Ki-67 2 hours after BrdU injection, as expected if Ki-67 is expressed by all dividing cells (Scholzen and Gerdes, 2000; Kee et al., 2002).”
Cellular and Molecular Characterization of Multipolar Map5-Expressing Cells: A Subset of Newly Generated, Stage-Specific Parenchymal Cells in the Mammalian Central Nervous System (2013, background): “cells, with a peak during DNA synthesis and mitosis [57]) were consistently negative (Fig.”
Coupled Proliferation and Apoptosis Maintain the Rapid Turnover of Microglia in the Adult Brain (2017, background): “This means the labeling of Ki67 is approximately two times higher than that of BrdU (Kee et al., 2002), which only labels the S phase, comprising 50% of the duration of the cell cycle (Cameron and Greulich, 1963).”
Structural Plasticity and Hippocampal Function (2010, background): “Even at high doses, BrdU does not have cytotoxic effects and does not alter cell proliferation rates (Cameron & McKay 2001, Hancock et al. 2009, Kee et al. 2002).”
The role of neurotrophic factors in adult hippocampal neurogenesis, antidepressant treatments and animal models of depressive-like behavior (2007, methodology): “One limitation of this study is that Ki-67 only labels proliferating cells.”
Developmental profile of neurogenesis in prenatal human hippocampus: An immunohistochemical study (2014, background): “Ki-67 is thought to represent a more reliable marker for identifying cells that re-enter the cell cycle than PCNA (Kee et al., 2002).”
Epigenetic Modulation of Seizure-Induced Neurogenesis and Cognitive Decline (2007, methodology): “To determine the phenotype of dividing cells mediating the KA-induced increase in cell proliferation in the presence or absence of VPA, we analyzed proliferating progenitors (using Ki67 as a marker of cycling cells) (Gerdes et al., 1983; Kee et al., 2002) for their coexpression of DCX, a marker for ”
Extreme sensitivity of adult neurogenesis to low doses of X-irradiation. (2003, background): “antigen that is expressed during all stages of the cell cycle except G0 (35, 36).”
Reversible effect of X‐irradiation on proliferation, neurogenesis, and cell death in the dentate gyrus of adult mice (2007, neutral): “…adult mice with a single low dose at a low dose-rate, and analyzed proliferation and neurogenesis using endogenous markers for proliferation (Ki67: Kee et al., 2002; Scholzen and Gerdes, 2000) and dividing and differentiating cells of neuronal lineage (doublecortin: Couillard-Despres et al.,…”