Short‐term and long‐term survival of new neurons in the rat dentate gyrus

By A. Dayer, Abigail A. Ford, K. Cleaver, M. Yassaee, Heather A. Cameron. The Journal of comparative neurology, 2003

653 citations recorded by the source corpus.

New neurons continue to be generated in the dentate gyrus throughout adulthood. Previous studies have shown that a significant proportion of new granule cells labeled with the thymidine analogue bromodeoxyuridine (BrdU) are lost from the adult dentate gyrus within 2 weeks. How long this loss continues and the extent to which it represents cell death, as opposed to dilution of label, is unclear. To address these questions, adult rats were injected with BrdU, and BrdU labeling in the dentate gyrus was compared at several survival time points. Double labeling with BrdU and the cell cycle marker Ki‐67 showed that BrdU is detectable for up to 4 days in some cells that continue to divide, indicating that any decrease in the number of BrdU‐labeled cells after 4 days is likely to reflect cell death rather than BrdU dilution. Death of new cells in the granule cell layer occurred at a steady rate between 6 and 28 days after labeling, resulting in loss of 50% of BrdU‐labeled cells over this 22‐day period. New granule cells that survived this first month lived for at least 5 additional months. In contrast, 26% of the granule cells labeled with BrdU at the peak of dentate gyrus development on postnatal day (P) 6 died between 1 and 6 months after labeling. These findings suggest that granule cells born during adulthood that become integrated into circuits and survive to maturity are very stable and may permanently replace granule cells born during development. J. Comp. Neurol. 460:563–572, 2003. Published 2003 Wiley‐Liss, Inc.

Subfields: Cellular and Molecular Neuroscience, Developmental Neuroscience, Neurology

View Paper: https://www.semanticscholar.org/paper/f6eeb9509eeeecf2c472fd15cfdddd914646f06a

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Citations in the corpus

Cell types, lineage, and architecture of the germinal zone in the adult dentate gyrus (2004, result): “This finding is consistent with previous studies showing that more than half of the newly formed neurons die within the first month after their birth in the SGZ (Dayer et al., 2003; Kempermann et al., 2003).”
Experience-Specific Functional Modification of the Dentate Gyrus through Adult Neurogenesis: A Critical Period during an Immature Stage (2007, background): “The increased proliferation may be particularly relevant in group 1, because large numbers of BrdU-labeled cells continue to divide for at least a few days after BrdU injections (Dayer et al., 2003).”
Enhanced synaptic plasticity in newly generated granule cells of the adult hippocampus (2004, background): “In this time period the fate of the newly generated neurons (survival versus cell death) is determine”
Increasing Adult Hippocampal Neurogenesis is Sufficient to Reduce Anxiety and Depression-Like Behaviors (2015, background): “Although the role of BAX in LTD was not examined in dentate granule Figure 3 Genetic ablation of Bax in neural stem cells and progenitors protects against chronic CORT-induced reduction in adult hippocampal neurogenesis.”
Reversible effect of X‐irradiation on proliferation, neurogenesis, and cell death in the dentate gyrus of adult mice (2007, neutral): “During the first month of their life, new neurons are most susceptible to cell death, and only a small fraction of them is integrated permanently in the granular layer (Biebl et al., 2000; Dayer et al., 2003).”
GABA-CREB signalling regulates maturation and survival of newly generated neurons in the adult hippocampus (2009, background): “Neurons that have survived this period are incorporated for an extended time into the hippocampal network (Dayer et al., 2003; Kempermann et al., 2003; Kee et al., 2007; Tashiro et al., 2007).”
Delayed Development of Adult-Generated Granule Cells in Dentate Gyrus (2006, background): “One caveat of BrdU incorporation is that subsequent generations of newborn cells can be detected (Dayer et al., 2003).”
Antidepressants increase neural progenitor cells in the human hippocampus (2009, background): “In rats, BrdU co-localizes with Ki-67 over a 4-day window, making the colocalization timeframe specific (Dayer et al, 2003).”
Learning Enhances the Survival of New Neurons beyond the Time when the Hippocampus Is Required for Memory (2004, background): “One week later, at a time when many of these cells die (Cameron et al., 1993; Dayer et al., 2003), groups of animals were either trained on various learning tasks or not trained.”
Doublecortin expression levels in adult brain reflect neurogenesis (2005, background): “Interestingly, the eliminated supernumerary cells belong mainly to the population of progenitor cells and young neurons (Petreanu & Alvarez-Buylla, 2002; Winner et al., 2002; Dayer et al., 2003).”
Recruitment of adult-generated neurons into functional hippocampal networks contributes to updating and strengthening of spatial memory (2009, background): “During the second week after birth, new hippocampal cells enter a period during which they show enhanced synaptic plasticity, their functional maturation occurs, and their ultimate survival is determined (8, 9).”
Adult neurogenesis: from precursors to network and physiology. (2005, background): “In the rodent DG, the vast majority of newly born cells are postmitotic and at least partially differentiated between 3 and 7 days (111, 416), since during this period BrdU-labeled cells express NeuroD (417).”