The potential and challenges of nanopore sequencing

By David Deamer, A. Marziali, H. Bayley, Steven A. Benner, Tom Z Butler, M. Di Ventra, D. Branton, S. Garaj, A. Hibbs, Xiaohua Huang and 16 more. Nature Biotechnology, 2008

2,436 citations recorded by the source corpus.

A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore. The nanopore provides a highly confined space within which single nucleic acid polymers can be analyzed at high throughput by one of a variety of means, and the perfect processivity that can be enforced in a narrow pore ensures that the native order of the nucleobases in a polynucleotide is reflected in the sequence of signals that is detected. Kilobase length polymers (single-stranded genomic DNA or RNA) or small molecules (e.g., nucleosides) can be identified and characterized without amplification or labeling, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility. Further research and development to overcome current challenges to nanopore identification of each successive nucleotide in a DNA strand offers the prospect of 'third generation' instruments that will sequence a diploid mammalian genome for ∼$1,000 in ∼24 h.

Subfields: Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/abef08fb5692b784abf47008d13b30dc4c07f9d5

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Citations in the corpus

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Nanopore-based Fourth-generation DNA Sequencing Technology (2015, background): “Many experiments have focused on measuring the transverse tunneling current or capacitance when a single ssDNA molecule is driven through a solid-state nanopore with embedded electrodes [7,45,61,62].”
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ChIP-Seq: advantages and challenges of a maturing technology (2009, background): “If the 'third-generation' sequencing technologies that are under development deliver as promised, they will lead to another epoch of genome-scale investigation”
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