A window into third-generation sequencing.

By Eric E. Schadt, Steve W Turner, Andrew Kasarskis. Human Molecular Genetics, 2010

927 citations recorded by the source corpus.

First- and second-generation sequencing technologies have led the way in revolutionizing the field of genomics and beyond, motivating an astonishing number of scientific advances, including enabling a more complete understanding of whole genome sequences and the information encoded therein, a more complete characterization of the methylome and transcriptome and a better understanding of interactions between proteins and DNA. Nevertheless, there are sequencing applications and aspects of genome biology that are presently beyond the reach of current sequencing technologies, leaving fertile ground for additional innovation in this space. In this review, we describe a new generation of single-molecule sequencing technologies (third-generation sequencing) that is emerging to fill this space, with the potential for dramatically longer read lengths, shorter time to result and lower overall cost.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/3851872363740515cf56ac5ac6955b10f29a7f61

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Citations in the corpus

Culture-independent molecular tools for soil and rhizosphere microbiology (2013, neutral): “kinetic information [159]”
TGS-GapCloser: A fast and accurate gap closer for large genomes with low coverage of error-prone long reads (2020, neutral): “…enabled this dramatic increase in genome sequencing, recent state-of-the-art techniques, such as third-generationsingle-moleculelongreads(TGS)[2,3],syntheticlong read (SLR) libraries [4–6], Hi-C [7], and BioNano physical maps [8], have provided long-range genome information to help increase…”
Sequencing technologies and genome sequencing (2011, methodology): “This sequencing from a single DNA molecule is now called as the “third generation of HT-NGS technology” (Schadt et al. 2010).”
Diagnosis of plant diseases using the Nanopore sequencing platform (2018, background): “advantages over the second generation by: (i) producing long read lengths, which are easier to map to a reference genome and to facilitate de novo assembly; (ii) fast run times (within a few hours); and (iii) requiring only a small amount of DNA with low cost for a single run (Schadt et al., 2010).”
Computational tools for copy number variation (CNV) detection using next-generation sequencing data: features and perspectives (2013, background): “Longer reads will greatly ease read alignment and CNV detection in repetitive regions of genome [75,76].”
Oxford Nanopore MinION Sequencing and Genome Assembly (2016, background): “…in TGS platforms: (1) increase in read length from tens of bases to tens of thousands of bases per read; (2) reduction of sequencing time from days to hours (or to minutes for real-time applications); and (3) reduction or elimination of sequencing biases introduced by PCR ampliﬁcation [8].”
The sequence of sequencers: The history of sequencing DNA (2016, background): “There is considerable discussion about what de ﬁ nes the different generations of DNA sequencing technology, particularly regarding the division from second to third [73,74,75,76].”
Genes and mutations causing retinitis pigmentosa (2013, background): “However, it is likely that whole-genome sequencing will become routine in the near future, especially with development of ‘third generation’ technologies (40).”
Environmental DNA - An emerging tool in conservation for monitoring past and present biodiversity (2015, background): “On the other hand, the DNA metabarcoding approach is powerful and cost-efficient (Thomsen et al., 2012a, 2012b; Yoccoz et al., 2012) and the technology for mass DNA sequencing continues to improve (Schadt et al., 2010; Shokralla et al., 2012).”
PacBio Sequencing and Its Applications (2015, methodology): “Unlike SGS, PacBio sequencing is a method for real-time sequencing and does not require a pause between read steps [1].”
Marker-Assisted Plant Breeding: Principles and Practices (2015, methodology): “At present, the third-generation DNA sequencing (TGS) methods are becoming commercially available; these methods sequence single DNA molecules without any cloning (Schadt et al. 2010).”