Processing of gene expression data generated by quantitative real-time RT-PCR.

By P. Y. Muller, Harald Janovjak, A. Miserez, Z. Dobbie. BioTechniques, 2002

1,514 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/ab0a3bb2e0e5c6d66521b2ba8d1f1ed593e1cb8b

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Citations in the corpus

• Total replacement of fish meal with plant proteins in diets for Atlantic cod (Gadus morhua L.) II — Health aspects (2007, methodology): “Mean normalized expression was calculated as (Eref) CT ref, /(Etarget) CT target, mean where Eref is the PCR amplification efficiency of the reference gene, Etarget is the PCR amplification efficiency of the target gene, CTref, mean is the threshold cycle of the PCR amplification for the reference g”
• Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations (2004, methodology): “For a subsequent data processing, the CP datasets can be imported into analysis software tools such as REST (Pfaffl et al. 2002), GeNorm (Vandesompele et al. 2002) or Q-Gene (Muller et al. 2002).”
• The tissue-specific lncRNA Fendrr is an essential regulator of heart and body wall development in the mouse (2013, methodology): “Quantification was calculated using the ΔΔCt method (Muller et al. 2002).”
• Total replacement of fish meal with black soldier fly (Hermetia illucens) larvae meal does not compromise the gut health of Atlantic salmon (Salmo salar) (2020, methodology): “The mean normalized expression of the target genes was calculated from raw Cq values (Muller et al., 2002).”
• Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, unknown): “, 1996) constitutive markers, respectively, for peach and Arabidopsis, was elaborated by the Q-Gene program (Muller et al., 2002).”
• Q-Gene: Processing Quantitative Real-time RT-PCR Data (2003, methodology): “Both of these procedures are provided by the software package Q-Geneand regarded as equivalent alternatives ( Muller et al., 2002).”
• Saturated fatty acids induce endoplasmic reticulum stress and apoptosis independently of ceramide in liver cells. (2006, methodology): “Reactions were run in triplicate and data calculated as the change in cycle threshold (∆CT) for the target gene relative to the ∆CT for β2-microglobulin and cyclophilin (control genes) according to the procedures of Muller et al (28).”