Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica

By G. Li, C. Quiros. Theoretical and Applied Genetics, 2001

1,570 citations recorded by the source corpus.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/aaa9e1c90f056b4247b4484e83cc963466dc1dc0

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In Molecular Biology

1. Milee Agarwal
2. H. Padh
3. Neeta Shrivastava
4. M. Truco
5. O. Ochoa
6. Jinguo Hu
7. R. Ahmad
8. B. Vick

In Plant Science

1. Milee Agarwal
2. H. Padh
3. Neeta Shrivastava
4. R. Graybosch
5. M. Truco
6. O. Ochoa
7. Jinguo Hu
8. R. Ahmad

Citations in the corpus

• Construction of an intra-specific sweet cherry (Prunus avium L.) genetic linkage map and synteny analysis with the Prunus reference map (2008, methodology): “Two sequence-related amplified polymorphism (SRAP) forward and reverse primer combinations me1–em1 and me1–em2 were used as reported by Li and Quiros (2001) ; me1=5′-TGAGTCCAAACCGGATA, em1=5′-GACTG CGTACGAATTAAT, em2=5′-GACTGCGTACGAAT TTGC.”
• Diversity and relationships among Turkish okra germplasm by SRAP and phenotypic marker polymorphism (2007, background): “SRAP markers are more consistent and repeatable than RAPDs, and are less labor-intensive and time-consuming to produce than amplified-fragment length polymorphism techniques (Welsh & McClelland 1990; Li & Quiros 2001; Ferriol et al. 2003; Budak et al. 2004a,b,c; Gulsen et al. 2005).”
• A new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers (2010, methodology): “…the stock and diluted portions were stored at -20 C. Primers Two-hundred and eight combinations of 13 forward and 16 reverse primers as studied by Li and Quiros (2001) and Gulsen et al. (2005); 12 POGP primer combinations designed to amplify members of peroxidase gene families and reported to…”
• Start Codon Targeted (SCoT) Polymorphism: A Simple, Novel DNA Marker Technique for Generating Gene-Targeted Markers in Plants (2009, neutral): “These techniques include inter retrotransposon amplified polymorphism, retrotransposon microsatellite amplified polymorphism (Kalendar et al. 1999), sequence-related amplified polymorphism (Li and Quiros 2001), and target region amplified polymorphism (TRAP; Hu and Vick 2003).”
• A intervarietal genetic map and QTL analysis for yield traits in wheat (2007, methodology): “The sequences of other polymorphic ISSR primers in ChSh population were listed in Table 1, and SRAP and TRAP primer pairs in Table 2 and Table 3 ( Li and Quiros 2001; Ferriol et al. 2003; Hu and Vick 2003; Li et al. 2003).”
• Molecular evidence for a single genetic clone of invasive Arundo donax in the United States (2008, methodology): “Originally, the SRAP technique was developed to preferentially amplify open reading frames (ORFs) using combinations of two different primers each 17 or 18 nucleotides long with AT- and CG-rich core sequences (Li and Quiros, 2001).”
• Advances in molecular marker techniques and their applications in plant sciences (2008, methodology): “combines simplicity, reliability, moderate throughput ratio and facile sequencing of selected bands (Li and Quiros 2001).”
• Target Region Amplification Polymorphism (TRAP) for Assessing Genetic Diversity in Sugarcane Germplasm Collections (2006, background): “primers supposedly target introns and exons, respectively (Li and Quiros, 2001).”
• DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing (2018, methodology): “Li and Quiros [42] developed this method mainly for the amplification of open reading frames (ORFs).”
• Target region amplification polymorphism: A novel marker technique for plant genotyping (2003, methodology): “J:\PMBR\Pmbr 2103 sep\R03-022.vp November 24, 2003 9:20:24 AM Primer design, arbitrary primers We adapted the concept of Li and Quiros (2001) to design the arbitrary primers.”